异丙酚对大鼠内毒素性脑损伤的影响

Effects of propofol on lipopolysaccharide-induced brain injury in rats

目的评价异丙酚对大鼠p1毒素性脑损伤的影响。方法健康清洁级SD大鼠54只，周龄6周，体重200-250g，采用随机数字表法，将大鼠随机分为3组：对照组（c组，n=6）、脂多糖（LPS）组（L组，n=24）和异丙酚组（P组，n=24）。L组和P组由左颈内动脉注射LPS1mg／kg制备内毒素性脑损伤模型，P组注射LPS后即刻腹腔注射异丙酚100mg／kg，L组腹腔注射等容量生理盐水，c组分别于左颈内动脉和腹腔注射与LPS和异丙酚等容量生理盐水。c组于腹腔给药后24h时，L组和P组分别于腹腔给药后6、24、48和72h时随机处死6只大鼠，取脑组织，测定脑含水量、高迁移率族蛋白1（HMGB1）表达和NF—tcB活性，光镜下观察脑组织病理学结果。脑含水量与HMGB1表达、NF—kB活性间进行直线相关分析。结果与c组比较，L组脑含水量升高，HMGBI表达上调，NF-kB活性升高（P〈0．05）；与L组比较，P组脑含水量降低，HMGB1表达下调，NF-kB活性降低（P〈0．05）；P组脑组织病理学损伤程度轻于L组；脑含水量与HMGB1表达、NF-kB活性呈正相关（r分别为0．692和0．769，P〈0．05）。结论异丙酚可减轻大鼠内毒素性脑损伤，其机制与减轻脑组织炎性反应有关。

Objective To investigate the effects of propofol on lipopolysaccharide （LPS）-induced brain injury in rats.Methods Fifty-four pathogen-f-ee SD rats of both sexes, aged 6 weeks, weighing 200-250 g, were randomly divided into 3 groups： control group （group C, n = 6） ; LPS group （group L, n = 24） ; propofol group （group P, n = 24）. Brain injury was produced by injection of LPS 1 mg/kg via the left internal carotid artery in L and P groups. Propofol 100 mg/kg was injected intraperitoneally immediately after the LPS administration in group P, while the equal volume of normal saline was given instead of propofol in group L. The equal volume of normal saline was given instead of LPS and propofol in group C. Six rats in each group were sacrificed and the brain tissues were immediately removed at 24 h after intraperitoneal administration in group C, and at 6, 24, 48 and 72 h after intraperitoneal administration in L and P groups for determination of brain water content, high-mobility group box 1 （HMGB1） expression and NF-kB activi.y, and microscopic examination. Results The brain water content and NF-kB activity were significantly increased, and HMGB1 expression was up-regulated in group L as compared to group C （P 〈 0.05）. The brain water content, expression of HMGB1 and NF-kB activity were significantly lower in group P than in group L （ P 〈 0.05）, The microscopic examination showed that brain injury was attenuated in group P compared with group L. The brain water content was positively correlated with the HMGB1 expression and NF-kB activity （ r = 0.692 and 0. 769 respectively, P 〈 0.05 ）. Conclusion Propofol can reduce the LPS-induced brain injury by reducing inflammatory response of the brain tissues.