摘要
目的 探讨异氟醚预先给药对缺氧复氧诱发的人脐静脉内皮细胞(HUVECs)多配体蛋白聚糖.1脱落的影响。方法HUVECs培养于EMB-2培养液,采用随机数字表法,将其随机分为3组(n=32),正常对照组(C组):密闭箱内逗人5%cq.95%02混合气体30min,在正常氧浓度下继续培养;缺氧,复氧组(H/R组)置于氧浓度为1%缺氧培养箱内孵育4h后,置于正常氧浓度的培养箱复氧2h;异氟醚预先给药组(I组):密闭箱内通入1.73%异氟醚,30min后行细胞缺氧复氧,方法同H/R组。缺氧复氧结束时测定HUVECs多配体蛋白聚糖.1表达、培养液脱落多配体蛋白聚糖-1浓度、细胞渗透性和细胞活力。结果与C组比较,H/R组和I组培养液脱落多配体蛋白聚糖-1浓度和细胞渗透性升高,HUVECs多配体蛋白聚糖-1表达水平和细胞活力降低(P〈0.01);与H/R组比较,I组培养液脱落多配体蛋白聚糖-1浓度和细胞渗透性降低,HUVECs多配体蛋白聚糖-1表达水平和细胞活力升高(P〈0.01)。结论异氟醚预先给药可通过抑制HUVECs多配体蛋白聚糖-1的脱落,从而减轻HUVECs缺氧复氧损伤。
Objective To investigate the effect of isoflurane pretreatment on hypoxia/reoxygenation (H/R)-indueed syndecan-1 shedding from hunan umbilical vein endothelial cells (HUVECs) .Methods HUVECs were cultured in EMB-2 medium and randorrly divided into 3 groups ( n = 32 each) : control group (group C), H/R group and isoflurane pretreatment group (group I). H/R was produced by 4 h exposure of HUVECs to hypoxia followed by 2 h reoxygenation in H/R and I groups. HUVECs were exposed to the mixture of 5% CO2 and 95% O2 for 30 min and then cultured in normal cultme atmosphere (21% O2 ) in group C. In group I, HUVECs were exposed to 1.73% isoflurane and incubated for 30 min before H/R. The syndecan-1 expression, concentrations of shed syndecan-1 in the medium, and cell permeability and viability were measured at the end of reoxygenation. Resuits Compared with group C, the shed syrdecan-I concentration in the medium and cell permeability were significantly increased, while the syndecan-I expression and cell viability decreased in H/R and I groups (P 〈 0.01 ). Compared with group H/R, the shed syndecan-1 concentration in the medium and cell permeability were significantly decreased, while the syndecan-1 expression and cell viability increased in group I (P 〈 0.01 ). Conclusion Isoflurane pretreatment can protect HUVECs against H/R injury through inhibiting the syndecan-1 shedding.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第5期627-629,共3页
Chinese Journal of Anesthesiology