α-颗粒膜蛋白在猪皮肤撕脱伤血栓形成中的作用

Effect of GMP-140 on thrombosis after skin avulsion injury in pigs

目的：探讨皮肤撕脱伤后α－颗粒膜蛋白的变化及在血栓形成中的作用．方法：①体内部分：将猪分成对照组和实验组，采用放免法测定血小板表面和血浆中α－颗粒膜蛋白的含量．②体外部分：离体培养人脐静脉内皮细胞，通过放免法测定组胺作用下内皮细胞与白细胞之间的粘附力及ＧＭＰ－１４０单抗对其影响．结果：皮肤撕脱伤后猪血小板表面及血浆中α－颗粒膜蛋白含量增加（Ｐ＜０．０５），内皮细胞与白细胞的粘附力因α－颗粒膜蛋白含量的增加而增加，但可以被ＧＭＰ－１４０单抗消除．结论：皮肤撕脱伤后血中α－颗粒膜蛋白的含量增加参与了撕脱后早期血栓的形成．

AIM: To study the concentration of GMP 140 and the effect on thrombosis after skin avulsion injury.METHODS: ① The pigs were divided into two groups of control and avulsed skin flaps in vivo . GMP 140 concentration of platelet surface and plasma was measured by radio immunoassay. ②EC was isolated from human umbilical cord in vitro . It was carried out by radioimmunoassay for the effect of histamine and GMP 140 antibody on leukocyte adhesion to endothelial cell. RESULTS: GMP 140 concentration of platelet surface and plasma in pig increased significally after avulsion ( P <0 05). With increase of GMP 140 concentration, leukocyte adhesion to endothelial also went up, but it can be stop by GMP 140 antibody. CONCLUSION: The change of GMP 140 concentration is involved in early thrombosis after skin avulsion.