RUNX3和Survivin在卵巢上皮性癌中的表达及其临床意义

The expression and clinical study of RUNX3 and survivin proteins in ovarian epithelial cancer

目的探讨RUNX3蛋白和Survivin蛋白在卵巢上皮性癌中的表达及其临床意义。方法用免疫组化SP法检测5例正常卵巢组织、10例良性卵巢上皮性肿瘤及50例卵巢上皮性癌组织中RUNX3蛋白和Survivin蛋白的表达情况。结果①在正常卵巢组织、卵巢良性囊性上皮性肿瘤及上皮性癌中RUNX3蛋白的表达率分别为80%、70%、28%,Survivin蛋白的表达率分别为0、10%、72%。在正常卵巢和良性卵巢肿瘤中RUNX3蛋白和Survivin蛋白的表达差异无显著性意义(P>0.05);而良性卵巢肿瘤和卵巢癌中RUNX3蛋白和Survivin蛋白的表达差异有显著性意义(P<0.05);②在卵巢癌中RUNX3蛋白和Survivin蛋白的表达与病理组织学分化程度及临床分期密切相关(P<0.05);③RUNX3蛋白和Survivin蛋白在卵巢癌组织中表达呈负相关。结论 RUNX3蛋白和Survivin蛋白可能共同参与了卵巢癌的发生、发展过程,二者的联合检测有可能成为判断卵巢癌临床侵袭及预后的分子标志。

Objective To study the expression and clinical relationship of RUNX3 and survivin proteins in human ovarian epithelial carcinoma.Methods Immunohistochemistry was used to detect the expressions of RUNX3 and survivin proteins in 5 cases of normal ovary,10 cases of benign epithelial tumor and 50 cases of ovarian epithelial carcinoma.Results ①The positive expression rates of RUNX3 in normal ovary,ovarian benign tumor and ovarian epithelial carcinoma were 80%,70%,28% respectively,while survivin expression rates in normal ovary,ovarian benign tumor and ovarian epithelial carcinoma were 0,10%,72% respectively.Comparison of the expression rates of RUNX3 and survivin proteins among groups showed that there was no significant difference between normal ovary and ovarian benign tumor（P0.05）,but there was statistically difference between ovarian benign tumor and ovarian epithelial carcinoma（P0.05）.②The expressions of RUNX3 and survivin proteins in ovarian epithelial carcinoma were closely related with pathological grade and clinical stage（P0.05）.③The expression of RUNX3 protein was negatively correlated with survivin protein in ovarian epithelial carcinoma.Conclusion RUNX3 and survivin proteins may play important roles together in the genesis and development of ovarian epithelial carcinoma.They may be used as new molecular markers for diagnosis and prognosis of ovarian epithelial carcinoma.