透明质酸刺激因子对增生性瘢痕成纤维细胞生长增殖的影响

The influence of HASF on the growth and proliferation of scar derived fibroblasts

目的：了解兔源性透明质酸刺激因子（ｈｙａｌｕｒｏｎｉｃａｃｉｄｓｔｉｍｕｌａｔｉｎｇｆａｃｔｏｒ，ＨＡＳＦ）对人增生性瘢痕成纤维细胞生长增殖的影响．方法：将ＨＡＳＦ作用于体外培养的增生性瘢痕成纤维细胞，测定３Ｈ－ＴｄＲ参入量、ＭＴＴ反应Ａ值及上清液中透明质酸（ＨＡ）的含量．结果：１×１０－３～１×１０－２ｇ／ＬＨＡＳＦ可剂量依赖性地使细胞３Ｈ－ＴｄＲ参入量及ＭＴＴ反应Ａ值降低，ＨＡ含量明显增加（Ｐ＜０．０１）．结论：兔源性ＨＡＳＦ在体外能促进人增生性瘢痕成纤维细胞合成ＨＡ，对其生长增殖有明显的抑制作用；这种抑制作用可能是通过ＨＡＳＦ刺激细胞合成ＨＡ来实现的．

AIM: To study the influences of hyaluronic acid stimulating factor (HASF) on the growth and proliferation of scar derived fibroblasts. METHODS: 3H TdR incorporation and MTT OD value was detected and the content of HA was determined with radioimmunology after the HASF was added to the monolayer culture system of scar derived fibroblasts. RESULTS: HASF at 1×10 -3 ～1×10 -2 g/L could decrease the 3H TdR incorporation and the MTT OD value in a dose dependent manner, and apparently increase the content of HA( P <0.01). CONCLUSION: HASF can promote scar derived fibroblasts to synthesize HA and inhibit its growth and proliferation in the monolayer culture system. This inhabitation may be caused by the increase of HA, which was synthesized by fibroblasts and stimulated by HASF.