植入知名血管束提高真皮下血管网皮片成活能力的动物实验

The animal experiment of the viability of subcutaneous vascular network(SVN) skin graft be improved by implanting an axial blood vessel

目的：提高真皮下血管网（ＳＶＮ）皮片的成活能力．方法：我们以１５ｋｇ～２０ｋｇ小猪为实验动物，建立了旋髂深动、静脉血管束植入ＳＶＮ皮片进行预构的动物模型．植入的血管束长１２ｃｍ，ＳＶＮ皮片９ｃｍ×６ｃｍ大小，０．３ｃｍ厚，皮下脂肪厚０．２ｃｍ．对１５头小猪在术后第１５日进行了皮片成活面积的统计，并采用荧光素钠法观察血管束血管化ＳＶＮ皮片的时程．结果：①实验侧成活面积百分率平均为８１．７％，对照侧成活面积百分率平均为３３．０％，Ｐ＜０．０５，二者差别有显著性，实验侧ＳＶＮ皮片的成活面积大于对照侧ＳＶＮ皮片的成活面积．②荧光素钠法证实，植入的知名血管束在术后３ｄ～５ｄ开始与ＳＶＮ皮片建立血运．结论：证实植入知名血管束进行ＳＶＮ皮片的预构，可以有效提高皮片的成活能力．

AIM: To improve the viability of subcutaneous vascular network(SVN) skin graft. METHODS: A new animal model was developed by implanting an axial arteriovenous boundle under subcutaneous vascular network (SVN) skin graft in a pig. The length of the axial blood vessel is 12 cm, and the size of SVN skin graft is 9 cm×6 cm, and the thickness of SVN skin graft is 0.3 cm,including 0.2 cm thickess of subcutaneous fat. The survival area of SVN skin graft was used to test 15 days postoperation in 15 pigs and using fluorescent tested the time of SVN skin graft being revascularized by axial blood vessel. RESULTS: ①The percent of survival area in experimental side average 81.7%, in control side, average 33.0%, P <0.05, the difference is significant.②The connection between the axial blood boundle and SVN skin graft begins 3 to 5 days after operation. CONCLUSION: Implanting an axial vessel under SVN skin graft can improve the survival of SVN skin graft.