胶原包埋聚羟基乙酸构筑血管模型

Vascular model with tubular polyglycolic acid scaffold precoated with collagen

目的：通过用交联胶原包埋处理聚羟基乙酸（Ｐｏｌｙｇ－ｌｙｃｏｌｉｃａｃｉｄ，ＰＧＡ），形成胶原加聚羟基乙酸管形支架，并接种牛血管内皮细胞于其内腔面，观察内皮细胞生长分化情况．方法：采用酶消化法从牛主动脉中分离培养牛血管内皮细胞并传代、纯化，将聚羟基乙酸无纺网用胶原溶液包埋，真空冷冻干燥，形成多孔状ＰＧＡ＋胶原管型支架；接种３代～７代牛血管内皮细胞于其内腔面，采用动态旋转培养技术体外培养１０ｄ，行扫描电镜观察．结果：该支架具有一定弹性和韧性，在培养液中，能较长时间保持形状．内皮细胞在其内表面形成较完整内皮细胞层．覆盖率约７２．６％±３．５％．结论：此支架可以作为组织工程技术并造血管的较理想支架之一．此方法为进一步应用组织工程方法构筑具有分层结构的人造血管打下基础．

AIM:The human always look for vessel′s best substitution. Using tissue engineering technique it is possible that we can culture “autogenous” vessel in vitro . METHODS: Seed bovine endothelial cell onto tubular porous polyglycolic acid scaffold precoating with collagen. Observe cell′s growth and differentiation on this scaffold. Bovine endothelial cells were collected from calf thoracic aorta by enzymatic digestion methods, then cultured, passaged and purified. Dip tubular porous polyglycolic acid scaffold into collagen acetic acid solution, then put it in an incubator at 37℃ to promote spontaneous gelation of collagen. 3 rd^7 th passages of bovine endothelial cells were seeded onto this material, cultured in vitro for 10 days using dynamic rotation culture technique. Scanning election microscopy was used to analyse. RESULTS: The scaffold has elasticity and tenacity, maintain its shape in culture medium. Endothelial cells form confluent monolayers on its inner surface, covering 72.6%±3.5% inner surface. CONCLUSION: tubular porous PLA precoating with collagen can be used as scaffold for constructing vessel in tissue engineering. This will be a good foundation for architecture of a hierarchically structured vessel in next stage.