全血中铅的石墨炉原子吸收光谱测定方法改进

Modification on Graphite Furnace Atomic Absorption Spectrometric Method for Determination of Lead in Whole Blood

目的建立一种操作相对方便,结果可靠的用石墨炉原子吸收光谱法测定全血中铅的方法。方法改进采集血样方式,改变基体改进剂,采用AA7020型石墨炉原子吸收光谱仪,寻求适合仪器的最佳操作条件,全自动进样方法进行测定。结果方法测血铅的重复性好,线性范围为3-100μg/L,相关系数为0.9997,RSD为2.6%-9.6%,回收率为93.2%-105.2%,方法的检出限为0.92μg/L。结论此方法具有结果准确可靠、快速简便、样品不需消化和污染小等优点,可适用于基层单位血铅的测定。

Objective To establish a graphite furnace atomic absorption spectrometric method with the advantages of relatively easy operation and reliable result for the determination of lead in whole blood.Methods The method of blood sample collection was improved and matrix modifier was changed.The best operation condition of the instrument was studied.The samples were automatically injected and the blood lead level was determined with graphite furnace atomic absorption spectrometer（AA7020）.Results The detection method had good repeatability.The linear range of determination was 3~100 μg/L.The correlation coefficient was 0.9997,and RSD was 2.6%~9.6%.The recovery rate was 93.2%~105.2%,and the detection limit was 0.92 μg/L.Conclusions This method has the advantages of accuracy,reliability,rapidness,simpleness,small pollution and without sample digestion.It can be applied to the determination of blood lead in grass-roots medical institutions.