胃原发癌和淋巴结转移癌细胞中bcl-2和c-myc基因蛋白表达的研究

bcl 2 and cmyc Oncoprotein Expression in Gastric Primary Carcinoma and Lymph Node Metastatic Carcinoma

为探讨ｂｃｌ２、ｃｍｙｃ 基因蛋白在胃原发癌与转移癌中的表达特点，应用ＳＰ免疫组织化学方法，对８０ 例胃癌、３５ 例相应淋巴结转移癌细胞进行ｂｃｌ２ 、ｃｍｙｃ 基因蛋白的检测，并对２ 种基因的生物学和临床病理意义进行研究和探讨。结果发现ｂｃｌ２ 、ｃｍｙｃ 基因蛋白的阳性表达率在３５ 例胃原发癌中分别为３１．４ ％ 、５７．１ ％ ，在淋巴结转移癌中分别为６８．６ ％ 、３１．４ ％ ，ｂｃｌ２ 在淋巴结转移癌中的表达率明显高于相应的原发癌（ Ｐ＜０ ．０１） ，而ｃｍｙｃ 阳性表达率明显低于相应的原发癌（Ｐ＜０ ．０５）。在８０ 例胃癌中，ｂｃｌ２、ｃｍｙｃ 的阳性表达率分别为４６ ．３％ 、６７．５％ ，其中分化较好者为６４ ．７％ 、７３．５％ ，分化较差者为３２．６ ％ 、６３ ．０％ ；肠型胃癌为６２．５％ 、７１．８％ ，弥漫型胃癌为３５ ．４％ 、６４ ．６％ ；无淋巴结转移的胃癌为６５ ．２ ％ 、６９ ．６ ％ ，有淋巴结转移者分别为３８．６％ 、６８．７％ 。结果提示：ｂｃｌ２、ｃｍｙｃ 基因蛋白的表达可能参与转移癌的形成；ｂｃｌ２、ｃｍｙｃ 基因蛋白的阳性表达与患者性别、年龄、肿瘤发生部位、浸润深度无关；

In order to investigate the expressions of bcl 2 and c myc oncoprotein in gastric primary carcinoma(GPC) and lymph node metastatic cancer(LMC),specimens from 80 gastric carcinoma(GC) and 35 corresponding local LMC were examined with streptavidin peroxidase immunohistochemical method.The expressions of bcl 2 and c myc and their biological and clinicopathologic significance were analyzed.The results showed that the positive rates of bcl 2 and c myc were 31.4%,57.1% in 35 case of GPC, 68.6% ,31.4% in LMC,respectively,the expression of bcl 2 in LMC was significantly higher than in GPC( P <0.01),the c myc expression in GPC was markedly higher than in LMC( P <0.05),the positive rates of bcl 2 and c myc were 46.3% ,67.5% in 80 cases of GC,64.7%,73.5% in well differentiated ones,32.6%,63.0% in pooly differentiated ones,62.5%,71.8% in intestinal type ones,35.4%,64.6% in diffuse type ones,65.2%,69.6% in GC without local lymph node metastasis,38.6%,68.7% in GC with local lymph node metastasis,respectively.The findings suggested that bcl 2 and c myc gene may be involved in the formation of LMC,the expressions of bcl 2 and c myc were independent of the sex and age of patients,the location of cancer and the invasion depth,the expression of bcl 2 was related to the degree of differentiation,Lauren types and with or without lymph node metastasis.However the relations did not showed to c myc expression.