原发性肝癌组织中Fas和FasL的表达研究

Fas and FasL Expression in Primary Hepatocellular Carcinoma

为了探讨原发性肝癌组织中乙肝病毒和丙肝病毒的感染与Ｆａｓ 和ＦａｓＬ表达的关系，采用免疫组化方法观察４０ 例原发性肝癌组织中Ｆａｓ、ＦａｓＬ、ＨＢｓＡｇ 及ＨＣＶ 抗原（ＮＳ５） 的表达。结果显示：癌细胞Ｆａｓ 阳性７ 例，阳性率为１７．５％ ，ＦａｓＬ阳性６例，阳性率为１５．０％ ；癌旁肝细胞Ｆａｓ 阳性２１ 例，阳性率５２ ．５％ ，ＦａｓＬ阳性１８ 例，阳性率４５ ．０％ 。癌组织中ＨＢｓＡｇ 阳性１２ 例，阳性率为３０．０％ ；癌旁组织中２５ 例阳性，阳性率为６２ ．５％ 。１２ 例ＨＢｓＡｇ 阳性的癌组织中，Ｆａｓ 阳性６ 例，ＦａｓＬ阳性５ 例；２８ 例ＨＢｓＡｇ 阴性的癌组织中，Ｆａｓ 和ＦａｓＬ 阳性各１ 例；癌组织中ＨＣＶＮＳ５ 阳性１１ 例，阳性率为２７ ．５ ％ ，癌旁组织中其阳性率为１２ ．５％（５／４０） ，１１ 例ＨＣＶＮＳ５ 阳性的肝癌组织中，Ｆａｓ 和ＦａｓＬ 阳性各５ 例，２９ 例ＨＣＶＮＳ５ 阴性的肝癌组织中，Ｆａｓ 阳性２ 例，ＦａｓＬ阳性１ 例。２ 组比较有显著性差异（ Ｐ＜０ ．０５） 。结果表明肝癌组织中Ｆａｓ 和ＦａｓＬ的表达与ＨＢＶ 和ＨＣＶ 的感染有一定关系。癌旁肝组织Ｆａｓ

Objective to study the relationship between Fas,FasL expression and hepatitis B virus and hepatitis C virus infection and their roles in hepatocarcinoma genesis.Methods expression of Fas,FasL,HBsAg and HCV NS5 antigen were examined in 40 cases of primary hepatocarcioma(HCC) by immunohistochemistry technique.Results 12 cases(30%) and 25 cases(62.5%) were HBsAg positive in tumor tissues and surrounding tissues respectively.7 cases(17.5%) were Fas positive and 6 cases(15.0%) FasL positive in cancer cells.While 21 cases(52.5%) were Fas positive and 18 cases(45.0%) FasL positive in adjacent tissue.Of 12 cases of HBsAg positive,6 cases and 5 cases were positive for Fas and FasL respectively;Of 28 cases of HBsAg negative,1 case was positive both for Fas and FasL.11 cases(27.5%) and 5 cases(12.5%) were positive for HCV NS5 in tumor tissues and surrounding tissues respectively.Of 11 cases of HCV NS5 positive,5 cases were Fas positive and 5 cases FasL positive;Of 29 cases of HCV NS5 negative,1 case was Fas positive and 1 FasL positive.The distribution of positive signals of Fas and FasL were not completely in accordance with that of HBsAg and HCV NS5.Conclusions Fas and FasL expression may be related to HBV and HCV infection.The significance of overexpression of Fas and FasL in surrounding tissues was unknown.