补肾壮骨合剂对小鼠肝组织病理学变化的影响

Effects of the Bushenzhuanggu misture on histopathological change of liver in mice

[目的]观察补肾壮骨合剂对小鼠的亚急性毒性,从临床安全用药角度评价对肝组织病理学变化的影响.[方法]选用昆明种小鼠,随机分成补肾壮骨合剂大(10.0g/kg)、中(5.0g/kg)、小(2.5g/kg)剂量组和对照组(等量生理盐水);灌胃给药3周,每日1次,观察各组肝组织病理学变化.[结果]肝组织切片HE染色观察见,补肾壮骨合剂大剂量组肝细胞可见中度水肿和脂肪变性,中剂量组出现少量肝细胞脂肪变性和轻度水肿,小剂量组与对照组肝细胞比较未见病理学变化.[结论]补肾壮骨合剂剂量为2.5g/kg时对小鼠无明显不良反应,剂量为10.0g/kg时对小鼠肝脏有损害,可引起肝细胞脂肪变性.

OBJECTIVE To study the subacute toxicity of the Bushenzhuanggu misture in mice and to evaluate the effects of histopathological change of liver in mice in order to safe medication of the Bushenzhuanggu misture in clinic.METHODS Kunming mice were randomly divided into four groups,groups of large（10.0 g/kg）,middle（5.0 g/kg） and small（2.5 g/kg） dose of the Bushenzhuanggu misture and control group（equivalent physiological saline）.The mice were performed by intragastric administration with the Bushenzhuanggu misture for three weeks one time per diem,and were sacrificed by anesthetization with injecting the chloral hydrate to peritoneal cavity,and histopathological changes of liver were observed in each group of mice.RESULTS Moderate edema and fatty degeneration were seen in group of large dose of the Bushenzhuanggu misture,and mild edema and fatty degeneration were seen in group of middle dose,and weren＇t seen the histopathological change in group of small dose as compared with control group.CONCLUSION The Bushenzhuanggu misture has no any significant side effects when the dose is 2.5 g/kg,and injurys the mice liver with fatty changes when the dose is 10.0 g/kg.