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Bcl-2抑制CD3∈分子介导的T淋巴细胞凋亡 被引量:8

Overexpression of Bcl-2 Inhibited T Lymphocyte Apoptosis Mediated by CD3∈ Molecule
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摘要 目的 研究Bcl-2过量表达对人JurkatT淋巴细胞凋亡的影响。方法 采用电穿孔法将bcl-2基因表达载体转染CD8ε阳性的人JurkatT淋巴细胞(TJK),获得稳定表达Bcl-2的细胞株(TJK/Bcl-2)后,用抗CD8单克隆抗体刺激和流式细胞仪检测细胞凋亡。结果 Bcl-2过量表达可显著抑制TJK细胞凋亡。结论 Bcl-2参与CD3ε介导的T淋巴细胞凋亡的调控。 To study the role of bcl 2 gene overexpression in T lymphocyte apoptosis mediated by CD8ε chimera molecule. Methods The cells expressed CD8ε chimera molecule were transfected with a bcl 2 expression vector. The cells with Bcl 2 overexperssion were selected by G418 and limited dilution, and then treated with anti CD8 antibody. The cell death was evaluated with flow cytometry. Results It was shown that there were only 2 6% of cells with Bcl 2 overexpression died by apoptosis. Comparing with Jurkat and TJK cells without Bcl 2 expression, in which there were 6 1% and 33.7% of cell death respectively, suggesting that Bcl 2 overexpression remarkbly inhibited apoptosis of the T lymphocytes mediated by CD8ε chimera molecule. Conclusions Bcl 2 overexpression could block T lymphocyte apoptosis mediated by CD3ε molecule and the mechanisms remained to be clarified in detail.
出处 《中国医学科学院学报》 CAS CSCD 北大核心 1999年第6期421-424,共4页 Acta Academiae Medicinae Sinicae
基金 中华医学基金 !(CMB) (99- 699) 国家自然科学基金! (3 94 70 160 )
关键词 BCL-2基因 过量表达 CD3ε T细胞 细胞凋亡 bcl-2 gene overexpression CD3∈ T lymphocyte apoptosis
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  • 1Ju S T,Nature,1995年,373卷,6513期,444页

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  • 1杨燕燕,青格勒,达来,张冠华,彭灿泉,邵凯,苏和,田瑛,王新奇,郭延华,乌达巴拉,周璇,曹贵方.Ghrelin对绵羊早期体外胚胎发育的影响[J].畜牧兽医学报,2014,45(6):926-931. 被引量:3
  • 2Ting-Jun FAN,Li-Hui HAN,Ri-Shan CONG,Jin LIANG.Caspase Family Proteases and Apoptosis[J].Acta Biochimica et Biophysica Sinica,2005,37(11):719-727. 被引量:100
  • 3王静,崔玉东.BCL-2家族蛋白对线粒体膜通透作用的调控和caspases的影响[J].中国畜牧兽医,2006,33(4):28-30. 被引量:7
  • 4王健东,潘星华.细胞凋亡调节的分子遗传机制[J].国外医学(遗传学分册),1996,19(3):116-121. 被引量:17
  • 5Eguchi Y, Ewert D , Tsujimoto Y. Isolation and characterization of the chicken bcl-2 gene .. expression in a variety of tissues including lymphoid and neuronal organs in adult and embryo [J]. Nucleic Acids Res, 1992,20(16):4187-4192.
  • 6Ines K, Renate W, Peter P, et al. Analysis of a regulatory element in the 5'-untranslated region of the bcl-2 gene[J]. FEBS Letters, 1997,406 : 31-32.
  • 7Jimenez G S ,Khan S H ,Stommel J M ,et al. P53 regulation by post- translational modification and nuclear retention in response to diverse stresses[J]. Oneogene, 1999,18 : 76561.
  • 8Korsmeyer S J . BCl-2 initiate a new category of oncogenes : r egulators of cell death. Blood,1992,80(4) :879-886.
  • 9Maja T, Markus C, Bernd K. Cloning and functional analysis of cDNA encoding the hamster bcl22 protein [J].Biochemical and Biophysical Research Communications, 2000,275 : 899 - 903.
  • 10Zamzami N, Brenner G, Marzo I. Permeability transition proe inapoptosis[J]. Biosci Rep, 1997,17 : 67.

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