IL-1β对小鼠垂体正常细胞和ACTH瘤细胞生长的影响

The Effect of Interleukin-1β on Cell Growth of Normal Mouse Pituitary Cells and ACTH Secreting Adenoma

目的 探讨垂体内自分泌、旁分泌因子IL-1β是否参与垂体细胞和垂体瘤细胞的生长增殖过程。方法 以体外原代培养的小鼠垂体正常细胞为对照,采用噻唑蓝(MTT)法观察10-9mol/L～10-13mol/LIL-1β对小鼠垂体ACTH瘤细胞(AtT20)生长增殖的影响。结果 (1)IL-1β刺激垂体原代培养细胞4～72h,不同浓度的IL-1β各组(n=9)与对照组(n=9)间的差异无显著性(P>0.05),实验中培养细胞处于对数生长期状态。(2)IL-1β刺激AtT20细胞48、72h,IL-1β各浓度组(n=9)细胞生长分裂的增加与对照组(n=9)相比差异有显著性(P<0.05,P<0.01)。结论 IL-1β参与了AtT20细胞生长增殖的调节,提示IL-1β可能是参与垂体ACTH瘤发病的因素之一。

To investigate whether IL 1β could affect the cell proliferation of normal pituitary cells and ACTH secreting adenoma cells. Methods Normal mouse pituitary cells were cultured in mininum essential medium D valine medium, a cultures virtually free of fibroblasts. AtT20 cells were also cultured as an ACTH secreting adenoma model. MTT method was used to study 10 -9 mol/L^10 -13 mol/L IL 1β effect on both normal and adenoma cells growth. Results (1) In the primate cells culture of normal mouse pituitary, IL 1β failed to influence cell growth during a period of 72 hours ( n=9, P>0 05 ). This finding can not confirm that IL 1β has no effect on pituitary cell growth, because of the variety of cell types in pituitary. (2) In the AtT20 cells, 10 -9 mol/L^10 -13 mol/L IL 1β stimulated cell proliferation significantly after 48 and 72 hours culturing respectively ( n=9, P<0 05, P<0 01 ). Conclusions These results suggested that IL 1β might be partially in involved in modulating pituitary adenoma cells growth.