β-胡萝卜素对白血病HL-60细胞凋亡及细胞周期分布的影响

EFFECTS OF β CAROTENE ON APOPTOSIS AND CELL CYCLE OF LEUKEMIC CELL LINE HL- 60

目的： 探讨β－胡萝卜素抑制白血病细胞增殖的机制。方法： 体外培养白血病细胞株ＨＬ－６０，培养时添加１０μｍ ｏｌ／Ｌ、５０μｍ ｏｌ／Ｌ、１００μｍ ｏｌ／Ｌβ－胡萝卜素作用２４ｈ、４８ｈ、７２ｈ 后，用台盼蓝拒染法进行活细胞计数，绘制细胞生长曲线；应用流式细胞术分析细胞周期分布，同时利用流式细胞术结合ＤＮＡ 凝胶电泳检测白血病细胞凋亡。结果： 细胞生长曲线显示１０～１００μｍ ｏｌ／Ｌ的β－胡萝卜素均对白血病细胞株ＨＬ－６０ 有显著性抑制作用，且呈剂量效应关系。流式细胞术结果显示５０μｍ ｏｌ／Ｌ的β－胡萝卜素处理ＨＬ－６０ 细胞２４ｈ 后，出现Ｓ期阻滞，并诱导５．５９％ 的细胞凋亡。ＤＮＡ 凝胶电泳结果同样显示β－胡萝卜素能够诱导白血病ＨＬ－６０ 细胞凋亡。结论： β－胡萝卜素对于髓系白血病细胞株ＨＬ－６０ 细胞的增殖具有显著性抑制作用，其机制可能是干扰的白血病细胞ＤＮＡ代谢；β－胡萝卜素诱导白血病细胞凋亡也是其抑制白血病的重要途径之一。

It is to study the mechanisms of inhibitory effects of β carotene on leukemic cell line HL 60. Methods: Leukemic HL 60 cells were cultured in vitro. Trypan blue exclusion, DNA gel electrophoresis and flow cytometric method (FCM) were used. Results: β carotene at concentrations 10～100μmol/L could inhibit the proliferation of myelogenous leukemic cell line HL 60 in dose dependent manner.FCM revealed that the growth of HL 60 cells was arrested in S phase after exposure to β carotene (50μmol/L). FCM and gel electrophoresis showed that the apoptosis of the leukemic cells could be induced by β carotene (50μmol/L). Conclusion: β carotene can inhibit proliferation of myelogenous leukemic cells. probably by interference of DNA metabolism and cell apoptosis.