大剂量吡哆醇的睾丸毒性体内外效应的比较研究

EFFECTS OF MEGADOSES OF PYRIDOXINE ON RAT TESTES IN VIVO AND IN VITRO

目的： 探讨大剂量吡哆醇（ＰＮ）的大鼠睾丸的毒性及可能机制。方法：采用腹腔注射和Ｓｅｒｔｏｌｉ－ｇｅｒｍ 细胞共培养两种模式，观察睾丸及副性腺变化。结果：注射１５ 天３００ｍ ｇ 和６００ｍ ｇ 组睾丸及副性腺萎缩，光镜和电镜下见精子细胞脱落、精母细胞变性坏死、精子释放延迟和异形精子，Ｓｅｒｔｏｌｉ细胞肿胀、微管稀少，内质网异位和扩张等。注射３０ 天效应更为明显。体外实验显示ＰＮ剂量与生精细胞脱落显著相关，并与Ｓｅｒｔｏｌｉ细胞骨架的变化一致。结论： 吡哆醇可能主要通过损伤Ｓｅｒｔｏｌｉ细胞，引起睾丸结构和功能改变。

To investigate the effects of pyridoxine (PN) on SD rat testes. Methods: Intraperitoneal injection and Sertoli germ cell co culture were carried out. Results: In addition to decreased weights of testes, epididymides, seminal vesicles and prostates, the testicular volumes were reduced too. Furthermore, exfoliation of the spermatids, degeneration or necrosis of the spermatocytes, sparseness of microtubules,dislocation and dilation of endoplasmic reticulum in Sertoli cells were observed under light and electron microscopes. Under the condition of PN exposure in vitro at different concentration and duration, the exfoliation of germ cells showed marked dose and time response relationships and damage in Sertoli cells showed a characteristic of loosing and retracting skeleton in the Sertoli germ cell co culture system. Conclusion: The toxic effects of PN on Sertoli cells may contribute to the constitutional and functional change of the rat testes.