硒和维生素E对糖尿病大鼠红细胞[Ca^(2+)]i水平的影响

THE EFFECT OF SELENIUM OR/AND VITAMIN E ON ERYTHROCYTE [Ca^( 2+)]i CONCENTRATION IN STZ INDUCED DIABETIC RATS

目的： 观察链脲佐菌素（ＳＴＺ）糖尿病大鼠补充硒（Ｓｅ）和／或维生素Ｅ（ＶＥ）后红细胞内游离钙浓度［Ｃａ２＋ ］ｉ的变化。方法： 用ＳＴＺ制成糖尿病（ＤＭ）大鼠，分为四组：（１）ＤＭ 对照组；（２）Ｓｅ 治疗组；（３）ＶＥ治疗组；（４）Ｓｅ 和ＶＥ治疗组；另有正常对照组，每天以同体积的生理盐水灌胃。实验期为３０ 天，分析血Ｓｅ、ＶＥ及红细胞［Ｃａ２＋ ］ｉ。 结果： 补充Ｓｅ 和／或ＶＥ后，红细胞［Ｃａ２＋ ］ｉ显著下降，与ＤＭ 对照组比较，差异有显著意义（Ｐ＜ ０．０５，Ｐ＜ ０．０１），以联合用药组降幅较大。血硒水平联合用药组高于其他各组（Ｐ＜ ０．０１），补Ｓｅ组高于补ＶＥ组和ＤＭ 对照组（Ｐ＜ ０．０１）；血清ＶＥ浓度联合用药组高于ＤＭ 对照组和补Ｓｅ 组（Ｐ＜ ０．０１，Ｐ＜ ０．０５），而补ＶＥ组血清ＶＥ浓度已恢复至正常水平。 结论： Ｓｅ和ＶＥ对ＤＭ 大鼠红细胞［Ｃａ２＋ ］ｉ异常升高有明显的抑制作用。

To study the change of erythrocyte intracellular free calcium concentration ([Ca 2+ ]i)in STZ induced diabetic rats supplemented with selenium (Se) and vitamin E (VE). Methods: The diabetes mellitus (DM) rats were prepared by STZ,then divided into 4 groups: (1)DM control, (2)Se treatments, (3)VE treatments, (4)Se,VE treatment. Another group was normal control, the same volume saline was given into the stomach every day. The experiment period was 30 days. The blood Se, serum VE and erythrocyte [Ca 2+ ]i were determined. Results:The erythrocyte [Ca 2+ ]i in diabetic Se or/and VE supplemented groups was significantly lower compared with that of DM control group (P< 0.01 or P< 0.05). The level of blood Se in DM+Se+VE group was higher than that of other groups (P<0.01), while the blood Se in DM+Se group was higher than that of DM+VE group and DM control group (P<0.01). Serum VE in DM+Se+VE group was higher than that of DM control and DM+Se group (P<0.01,P<0.05),while serum VE in DM+VE group was close to that of normal control rats. Conclusion: The results suggest that the supplementation of Se and VE inhibits significantly the increase of erythrocyte[Ca 2+ ]i.