抗氧化维生素对内皮细胞增殖及凋亡的影响

EFFECT OF ANTIOXIDANT VITAMINS ON PROLIFERATION AND APOPTOSIS OF AORTIC ENDOTHELIAL CELLS

目的： 观察氧化低密度脂蛋白（ｏｘＬＤＬ）对内皮细胞增殖与凋亡的影响以及抗氧化维生素（维生素Ｅ、维生素Ｃ及β－胡萝卜素）对内皮细胞的防治效应。方法： 在体外培养的小牛主动脉内皮细胞中分别加入不同浓度的抗氧化维生素，作用１２ｈ 后，再与终浓度为０．１ｇ Ｐｒ／Ｌ的ｏｘＬＤＬ共同培养２４ｈ，采用噻唑蓝比色分析法和流式细胞仪分析法对贴壁内皮细胞进行检测，分别观察抗氧化维生素对经ｏｘＬＤＬ作用的内皮细胞的形态、生长增殖、细胞周期及凋亡的影响。结果： （１）维生素Ｅ和维生素Ｃ能显著减轻ｏｘＬＤＬ对内皮细胞形态的损伤作用；β－胡萝卜素作用稍弱。（２）三种抗氧化维生素均可降低ｏｘＬＤＬ对内皮细胞生长增殖的抑制作用，使其抑制率降低。（３）三种抗氧化维生素均可促进内皮细胞由Ｇ１ 期进入ＤＮＡ合成的Ｓ期，促进内皮细胞的增殖并阻止内皮细胞凋亡的发生。结论： 维生素Ｅ、维生素Ｃ及β－胡萝卜素均可减轻ｏｘＬＤＬ在形态、增殖及凋亡等方面对内皮细胞的损伤作用，这可能是抗氧化维生素加速内皮细胞损伤后修复、促进内皮细胞增殖，从而发挥其抗动脉粥样硬化形成的部分机制。

It is to observe the effects of oxLDL on endothelial cell (EC) proliferation and apoptosis and to test the protective effects of Vit E,C and β carotene. Methods:Bovine aortic EC were incubated in culture media with vit E, C, and β carotene for 12h,and then exposed to oxLDL for 24h respectively.Results:(1)bovine aortic EC were deformed and shed as observed under phase contrast microscope in oxLDL group.The phenomena in antioxdant vitamin groups were slighter.(2)Inhibitory rate of bovine aortic EC proliferation in vit E,vit C and β carotene groups was significantly lower than that of oxLDL group. (3)Number of G 1 phase cells in oxLDL group was increased compared with vit E,vit C and β carotene groups respectively. (4)The apoptotic peak in oxLDL group was higher than that in antioxidant vitamin groups.Conclusion:Antioxidant vitamins have definite protective effects on injury induced by oxLDL on morphology,proliferation and apoptosis in EC possibly through promoting the demage repair and proliferation.