SM_2单克隆抗体的初步应用研究

The Initial Utilized of Monoclonal Antibody of Sulfamethazine

以磺胺二甲嘧啶(SM2)-人血清白蛋白(HSA)为免疫抗原和SM2-卵清白蛋白(OVA)为包被抗原。制备SM2单克隆抗体;利用杂交瘤技术和有限稀释法经亚克隆,得到三株特异性稳定分泌SM2抗体的杂交瘤细胞,与其他四种磺胺药和两种载体蛋白HSA、OVA均无交叉反应。利用本试验制备的单克隆抗体初步建立了动物组织中检测SM2的间接竞争ELISA方法。最低检出限为9.95 ng/mL,灵敏度为47.12 ng/mL。

Sulfamethazine-human serum albumin was used for the immunogen,and SM2-ovalbumin was used for the coating antigen,to make sulfamethazine monoclonal antibody.Using cell fusion and limiting dilution method to reclone to get three hybridoma strains,which could stable secret monoclonal antibody.No cross-reactivity was seen between the McAb and the other four sulfonamides,as well as the two carrier proteins.The monoclonal antibody was used to developed an indirect competitive inhibition enzyme linked immune absorbant assay.The limitation of SM2 determination was 9.95 ng/mL.The sensitivity was 47.12 ng/mL.