一种复方聚六亚甲基盐酸双胍消毒液消毒相关性能研究

STUDY ON DISINFECTION RELATED PROPERTIES OF A COMPOUND POLY HEXAMETHYLENE BIGUANIDE HYDROCHLORIDE DISINFECTANT

目的研究一种复方聚六亚甲基胍盐酸盐消毒液消毒相关性能。方法采用悬液定量杀菌试验和动物毒性试验的方法,对该复方消毒液杀菌效果及其毒性进行了实验室观察。结果用含聚六亚甲基盐酸双胍146 mg/L的该消毒液分别对悬液中的大肠杆菌、金黄色葡萄球菌、白色葡萄球菌及铜绿假单胞菌作用7.5 min,杀灭对数值均>5.00。用聚六亚甲基盐酸双胍146 mg/L的该复方消毒液对悬液内白色念珠菌作用22.5 min,杀灭对数值为2.07。该复方聚六亚甲基盐酸双胍消毒液对小鼠急性经口LD50值>5 000 mg/kg(体重);该消毒液原液对家兔眼睛黏膜和完整皮肤有轻刺激性和中等刺激性;该消毒液对小鼠骨髓嗜多染红细胞无致微核作用。结论该聚六亚甲基盐酸双胍消毒液对细菌繁殖体具有良好的杀灭效果,对小鼠属实际无毒级,无致微核作用,但对家兔眼睛和皮肤有一定刺激性。

Objective To study the disinfection related properties of a poly hexamethylene biguanide hydrochloride disinfectant.Methods Suspensionr quantitative germicidal test and animal toxic experiment were used to observe the germicidal efficacy and the toxicity of the compound disinfectant in laboratory.Results The killing logarithm values of Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa by the disinfectant solution containing poly hexamethylene biguanide hydrochloride 146 mg/L with 7.5 min contact time were all above 5.00.The killing logarithm value of Candida albicans and by the disinfectant solution containing poly hexamethylene biguanide hydrochloride 146 mg/L with 22.5 min contact time was 2.07.The LD50 of mice acute oral toxicity was 〉5 000 mg/kg·bw.The disinfectant has slightly-irritating to rabbit eye and mucosa and has medium irritating to rabbit skin.The result of mouse bone marrow polychromatic erythrocyte micronucleus test was negative.Conclusions The compound poly hexamethylene biguanide hydrochloride disinfectant had good germicidal efficacy to vegetative forms of bacteria.It was low toxicity and has slightly-irritating to rabbit eye and skin.