诊断超声对大鼠子宫内膜细胞凋亡的影响

THE EFFECT OF DIAGNOSTIC ULTRASOUND ON APOPTOSIS OF ENDOMETRIUMS CELL RATS

目的:探讨子宫内膜细胞凋亡与诊断超声持续照射时间的关系,以及生殖医学领域应用诊断超声所需安全照射时间。方法:将健康纯种二级Wistar雌性大鼠50只随机分为5组:空白对照组、B超照射10min组、20min组和30min组,彩超照射10min组;照射后24h取子宫内膜,采用流式细胞仪检测子宫内膜细胞早期凋亡的水平。结果:(1)流式细胞仪二维点图显示:对照组未见或偶见散在的早期凋亡细胞;B超照射10min组出现早期凋亡细胞;20min组及30min组的早期凋亡细胞明显增多,以30min组最为明显;彩超照射10min组出现较多的早期凋亡细胞;(2)早期凋亡率比较:照射各组与对照组比较,P<0.01,均有统计学意义,B超照射10min组与20min组及30min组之间均有统计学意义(P<0.01);相同频率彩超照射10min比B超照射10min早期凋亡率明显增高。结论:诊断超声辐照大鼠子宫可诱导内膜细胞凋亡增加,且凋亡率与超声持续时间及输出功率呈正相关。

Objective： To explore the relationships of endometrial apoptosis with continuing exposures of diagnostic ultrasound,and safe exposure of utilization diagnostics ultrasounds in the reproductive medicine.Methods： Fifty Wistar female Rats healthy purebreds second orders were randomLy divided intofive groups： blank group,experiment groups（exposure10min,20min,30min）,color Doppler ultrasound exposure 10min.The endometrium were obtained at 24h after exposure,Flow cytometry was used to detect the level prophases apoptosis of endometrial cells.Results：（1）The scatterplot two-dimensions was signify：blank group unemerge or perchance emerge sporadically prophase apoptosis;two-dimension ultrasonic radiation 10min occurrence prophase apoptosis;20min group and 30min group obviously increase,30min group the most obviously;color Doppler ultrasound 10min group appear major prophase apoptosis;（2） The rate ratio prophase apoptosis comparison： experiment groups with contrast,P0.01,two-dimension ultrasonic radiation 10min group and 20min group and 30min group contrast have statistical significance（P0.01）;The rate ratio of prophase apoptosis endometriums cells obviously heighten of ultrasonic radiation10min color Doppler ultrasound 10min with two-dimension ultrasonic radiation 10min.Conclusion： Diagnostic ultrasound may result in more uterus cells apoptosis in rats,output power and duration of ultrasound correlates significantly to apoptosis.