表没食子儿茶素没食子酸酯（EGCG）在活体内对鸟疟原虫生长抑制的研究

Growth Inhibitory Effects in vivo of Epigallocatechin-3-gallate （EGCG） on Avian Malarial Parasite

为了研究表没食子儿茶素没食子酸酯（Epigallocatechin-3-gallate，EGCG）在活体内对疟原虫的抑制，实验选取了感染虎纹伯劳Lanius tigrinus的伯劳疟原虫Plasmodium（Novyella）lanius作为研究对象。实验把通过血转种成功的50只带虫金丝鸟Serinus canaria分成5个组，每组10只进行实验。每组以不同剂量的EGCG进行灌喂实验，设蒿甲醚和生理盐水的阳性和空白对照，并用常规涂片法和PCR法进行抑制效果的检测。实验结果显示在不同剂量下，EGCG在体内对疟原虫生长的抑制率分别是66．7％、90％、90％。不同实验组与空白对照组有显著差异（P〈0．001），证实儿茶素在体内对鸟类疟原虫有抑制作用。

To explore the inhibitory effect of epigallocatechin-3-gallate （EGCG） on malarial parasite growth in vivo. Fifty canaries （Serinus canaria） , which were successfully inoculated with blood sample of a natural host, Lanius tigrinus infected by Plasmodium lanius, were assigned into 5 groups （ 10 each）. Canaries in group Ⅰ , Ⅱand Ⅲ were orally admitted EGCG 30. 0, 60.0 and 120.0 mg/ （kg · d）, respectively; in group IV and V were orally admitted artemether 50.0 mg/ （kg · d） or normal saline, separately. Results of Giemsa＇s staining and PCR examination showed the Inhibitive rates of EGCG in group Ⅰ , Ⅱ and Ⅲ were 66.7% , 90% and 90% , respectively; in group Ⅳ and V were 80% and 0, separately. Very significant differences existed between the tested groups （group Ⅰ , Ⅱ, Ⅲ and Ⅳ） and the control group （group V, P 〈 O. 001 ）, suggesting that EGCG played an inhibitive effect to Plasmodium （Novyella） lanius in Serinus canaria.