新疆伊犁那拉提草原啮齿动物感染恙虫病东方体调查

Investigation on Rodents Infection of Orientia tsutsugamushi in Some Areas of Nalati Grassland, Xinjiang Uygur Auto. Reg. , China

为了解新疆伊犁那拉提草原啮齿动物中自然感染恙虫病东方体（Ot）的情况，在那拉提草原捕获鼠和旱獭，分别取其脾组织提取DNA，应用巢式PCR（nPCR）方法检测Ot-Sta56基因；基因分型引物扩增阳性片段，判断该地区存在的0t基因型只，在其中3只鼠、2只旱獭的脾中检测到阳性片段，基冈型包括Karp型、Saitama型，BLAST结果显示其中1个片，部分阳性标本测序，通过NCBI网站对序列进行BLAST比较分析。结果显示，在该地区共捕获鼠67只，旱獭29段的核苷酸序列与Karp型同源性为97％，另1个片段未能测出核苷酸序列。推测在那拉提地区鼠类可能感染0t，基因型为Karp型，怀疑可能有Saitama型存在。

DNAs were extracted from spleens of the rodents captured from Nalati area of Xinjiang Uygur Auto. Reg. , and nested-polymerase chain reaction （nPCR） was performed to detect the Orientia tsutsugamushi （ Ot）-Sta 56 gene. Some positive samples were sequenced and analyzed by BLAST of NCBI network. A total of 67 rodents and 29 marmots were captured in Nalati area. The positive fragments were detected from the spleens of 3 mice and 2 marmots, including Karp and Saitama genotype. The detection rates of Ot from Mus musculus, Cricetulus migratorius and Marmota barbacina were 6.06% , 2.94% and 6.90% , respectively. The homology of one fragment of the amplified gene nucleotide sequence to Karp strain was 99%. The other fragments could not be sequenced. It was presumed that the Karp strain was the main gene type. and the Saitama strain might exist in this area.