塞来昔布联合放射对鼻咽癌细胞株凋亡的影响

Effects of celecoxib combined with radiotherapy on apoptosis of CNE-2Z cell lines

目的 探讨塞来昔布联合放射对人鼻咽癌细胞株CNE-2Z凋亡的影响及其可能的机制.方法 实验分4组进行,分别为对照组、药物组（25μmol/L塞来昔布）、照射组（8 Gy X线）和实验组（25 μmol/L塞来昔布＋8 Gy X线照射）.克隆形成实验检测塞来昔布对CNE-2Z细胞的放射增敏作用;流式细胞仪检测肿瘤细胞凋亡率;免疫细胞化学染色检测B淋巴细胞-2（Bcl-2）、凋亡活化基因（Bax）蛋白表达;Western blot技术检测Caspase-3蛋白表达.结果 塞来昔布对人鼻咽癌细胞CNE-2Z有放射增敏作用,实验组中CNE-2Z细胞平均存活分数为0.50,细胞平均致死剂量为2.36,与对照组比较差异有统计学意义（P值均＜0.01）;塞来昔布联合放射能够上调Bax的蛋白表达,对照组Box表达评分为1.221 ±0.116,实验组为2.758±0.256;同时抑制Bcl-2蛋白表达,对照组Bel-2表达评分为2.559±0.144,实验组为1.253±0.114,差异均有统计学意义（P值均＜0.05）;并明显增加肿瘤细胞的凋亡率（F=7.63,P＜0.01）,差异有统计学意义;Western blot结果显示实验组Caspase-3蛋白表达增强.结论 Cox-2抑制剂塞来昔布联合放射能诱导人鼻咽癌细胞株CNE-2Z凋亡,具有放射增敏作用.

Objective To investigate the effects of celecoxib combined with radiotherapy on apoptosis of CNE-2Z cell lines and the potential mechanisms.Methods Four groups were used,a control,celecoxib （25 μmol/L celecoxib） ,irradiation （ 8 Gy X ray） and celecoxib plus irradiation.The radiosensitising effect was detected by clone formation experiment.Flow cytometry was used to detect the apoptosis rate of cells.The expressions of Bcl-2 and Bax were assessed by immunocytochemistry.Western blot was used to examine the expression of Caspase-3.Results Celecoxib enhanced the radiosensitivity of CNE-2Z cells.In experimental group,the mean surviving fraction and the mean lethal dose of CNE-2Z cells were 0.50 and 2.36 respectively.Compared with the irradiated group,there was significant differences between the two groups （P ＜ 0.01 ）.Celecoxib combined with radiotherapy up-regulation the expression of Bax.The score of the expression of Bax in the control group and the experimental group were 1.221 ±0.116 and 2.758 ±0.256 respectively.Celecoxib combined with radiotherapy could inhibit the expression of the protein of Bcl-2.The score of the expression of Bcl-2 in the control group and the experimental group were 2.559 ± 0.144 and 1.253 ± 0.114 respectively,with significant differences （ P ＜ 0.01 ）.Celecoxib combined with radiotherapy cound increase the apoptosis rate of tumor cells with significant differences （ F =7.63,P ＜ 0.01 ）.Western blot showed that the expression of Caspase-3 was strengthened.Conclusion Celecoxib combined with radiotherapy could induce apoptosis and enhance the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cell lines.