自发性高血压大鼠发病过程中结缔组织生长因子表达的动态观察

Dynamic observation of connective tissue growth factor in pathogenic process of spontaneously hypertensive rats

目的：观察自发性高血压大鼠（SHR）发病过程中结缔组织因子（CTGF）的动态变化，探讨其与高血压病发生发展的关系。方法：采用RT—PCR检测心肌组织中CTGFmRNA的表达。用苏木精、伊红染色检测心肌纤维化。用免疫组化染色（SP法）检测CTGF在心肌组织中的分布。结果：随着鼠龄的增长，SHR组大鼠左室质量指数、CT-GF及其mRNA的表达均显著增加，其心肌细胞的大小也明显增大（P〈0．05）。SttR组大鼠心肌间质和血管平滑肌中，可见大量的呈棕黄色的CTGF阳性颗粒的分布，不同鼠龄组之间比较亦有显著性差异（P〈0．01）。结论：不同鼠龄（8—16周）的SHR大鼠心肌细胞中CTGF的表达显著增加，尤以16周龄的大鼠为著，同时伴有心肌纤维化进程加快，提示CTGF参与了高血压大鼠心肌病变的发生发展。

AIM： To observe the dynamic changes of connective tissue growth factor （CTGF） in the pathogenic process of spontaneously hypertensive rats （SHRs） and to explore the relationship between CTGF and the development of hypertensive cardiomyopathy. METHODS： RT-PCR was used to detect the expression of CTGF mRNA in myocardial tissues. Myocardial fibrosis was detected by hematoxylin and eosin staining and the distribution of CTGF in myocardial tissues was detected by immunohistochemical staining. RESULTS： With the increase in age, left ventricular weight index, CTGF and its mRNA expression significantly increased in SHR group. The size of cardiac myocytes also increased significantly in SHR group （P 〈 0. 05）. In the myocardial interstitial and vascular smooth muscle of the SHR group, a large number of brown CTGF positive particles were seen and the number was statistically significant between different age groups （P 〈 0. 01 ）. CONCLUSION： The CTGF expression in the myocardial tissue significantly increased in different age group of SHR （from 8 weeks to 16 weeks）, especially in the 16-week age group. Meanwhile, myocardial fibrosis progresses, suggesting that CTGF is involved in the occurrence and development of myocardial disease in hypertensive rats.