莪术醇对人鼻咽癌细胞CNE2增殖、凋亡及周期的影响

Effect of curcumol on proliferation,apoptosis and cell cycle of nasopharyngeal carcinoma CNE2 cells

目的探讨莪术醇(curcumol)在体外对人鼻咽癌CNE2细胞增殖、凋亡及周期的影响,为其临床治疗鼻咽癌提供理论依据。方法不同浓度莪术醇作用于人鼻咽癌CNE2细胞后,用MTT法和流式细胞仪检测不同时间点鼻咽癌细胞CNE2的增殖、凋亡及周期分布。结果莪术醇在体外明显抑制鼻咽癌CNE2细胞的增殖,且呈浓度和时间依赖性(P<0.05);不同浓度作用48 h后细胞凋亡率随浓度增加而增大,并使细胞周期阻滞于G2/M期。结论莪术醇能明显抑制鼻咽癌CNE2细胞增殖,诱导其凋亡,能将细胞阻滞于G2/M期,具有潜在的抗肿瘤作用。

Objective To study the effect of curcumol on proliferation,apoptosis and cell cycle of nasopharyngeal carcinoma（NPC） cell line CNE2 in vitro and to investigate the feasibility of curcumol for the treatment of NPC.Methods Nasopharyngeal carcinoma CNE2 cells were treaded with curcumol of different concentrations.The cell proliferation inhibition rates,apoptosis ratios and cell cycle status at different time-points were detected with MTT and flow cytometry.Results The CNE2 proliferation was inhibited in dose-dependent and time-dependent manners after treated by curcumol.48h after treatment,the apoptosis ratios showed increasing trends with the increased concentration of curcumol and CNE2 cells showed G2/M phase blockage with a dose-dependent way.Conclusion By inhibiting the proliferation of nasopharyngeal carcinoma CNE2 cells in vitro,inducing cell apoptosis and preventing the cell cycle in G2/M phase,curcumol has an anti-tumor potential.