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锌对砷中毒小鼠肝脏Fas和FasL表达影响的实验研究

The effects of zinc on the expression of liver Fas/FasL in arsenic poisoning mice
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摘要 目的 探讨砷对小鼠肝脏Fas、FasL表达的影响及锌的拈抗作用.方法 选用健康昆明种小鼠60只,按体质量分成5组:阴性对照组(不给砷也不给锌),单纯给砷组(55 mg/L NaAsO2溶液),砷+低锌组、砷+中锌组、砷+高锌组均给55 mg/L NaAsO2溶液,并分别给20、40、80 mg/L ZnSO4溶液.经口连续灌胃(按体质量灌胃量为0.02 ml/g.1次/d)6周,待实验结束后处死小鼠,采用免疫组化法测定小鼠肝脏巾凋亡因子Fas、FasL表达.结果 干预组随着给锌量的增加,肝脏Fas、FasL的表达率逐渐降低.低、中锌+砷组的Fas的表达率[83.33%(10/12)、50%(6/12)]、FasL表达率[66.67%(8/12)、41.67%(5/12)]与阴性对照组Fas[8.33%(1/12)]、FasL[0.00%(0/12)]表达率比较,差异有统计学意义(P均〈0.05);中、高锌+砷组的Fas的表达率[50%(6/12)、25%(3/12)]与阳性对照组[8.33%(1/12)]比较,差异有统计学意义(P均〈0.01),中、高锌+砷组的FasL的表达率[41.67%(5/12)、16.67%(2/12)]与阳性对照组[91.67%(11/12)]比较,差异有统计学意义(P均〈0.05).结论 砷可使小鼠肝脏Fas、FasL的表达率增高,促进肝脏的凋亡,锌可拮抗砷对细胞凋亡的作用. Objective To investigate the effects of arsenic on liver Fas/FasL expression in mice and to observe the antagonize effect of zinc. Methods Sixty health Kunming mice were divided into five groups according to their body mass: negative control group(no arsenic and no zinc), arsenic group(55 mg/L NaAsO2 solution), low dose zinc intervention group(55 mg/L NaAsO2 solution and 20 mg/L ZnSO4 solution), middle dose zinc intervention group (55 mg/L NaAsO2 solution and 40 mg/L ZnSO4 solution), and high dose zinc intervention group (55 mg/LNaAsO2 solution and 80 mg/L ZnSO4 solution). Everyday the solution was given by oral gavage at a dose of 0.02 ml/g body weight for six weeks. The expression of Fas/FasL in mice liver was examined by immunohistochemistry.Results With the amount of zinc increasing, the expression of both Fas and FasL in mice liver decreased gradually. The expression rates of Fas[83.33%(10/12), 50%(6/12)] and FasL[66.67%(8/12), 41.67%(5/12)]in low dose zinc intervention group and middle dose zinc intervention group, respectively, were different from the expression rate of Fas[8.33%(1/12)] and FasL[0.00%(0/12)] in the negative control group(all P 〈 0.05). The Fas expression rate of middle dose zinc intervention group[50%(6/12)] and the high dose zinc intervention group [25%(3/12)] was compared with the arsenic group[8.33%(1/12)], and the difference was statistically significant (all P 〈 0.01 ). The FasL expression rate of the middle dose zinc intervention group [41.67% (5/12 )] and the high dose zinc intervention group[16.67%(2/12)] was compared with positive control group[91.67%(11/12)], and the difference was statistically significant (all P 〈 0.05). Conclusions Arsenic can increase the expression of Fas and FasL in mice liver, and promote apoptosis in liver, zinc may antagonize the effect of arsenic.
出处 《中国地方病学杂志》 CAS CSCD 北大核心 2011年第4期403-404,共2页 Chinese Jouranl of Endemiology
关键词 抗原 CD95 Fas配体蛋白质 Zinc Arsenic Antigens, CD95 Fas ligand protein
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