乙型肝炎病毒大蛋白用于公共卫生体检领域检测意义的研究

The study on significance of detecting the hepatitis B virus large proteins in public health physical examination

目的 通过检测餐饮从业人员、学生等体检人员中乙肝病毒大蛋白（Hepatitis B virus large proteins,HBV-LP）的情况,探讨乙肝病毒大蛋白用于公共体检领域的意义.方法 分别采用酶联免疫法（ELISA）测定乙肝两对半（HBV-M）以及乙型肝炎病毒大蛋白（HBV-LP）,采用real-time PCR法检测HBV-DNA,对各类体检人员的709份标本分别进行检测.结果 （1）HBV-LP法与HBV-DNA 法检测709例中HBsAg阳性的体检人员的检测结果 差异无统计学意义,x2值和P值分别为1.47和0.23.（2）不同乙肝两对半模式中HBV-LP法与HBV-DNA法的检测结果 差异均无统计学意义,x2值和P值分别为2.67,0.60,0.00和0.10,0.44,1.00;（3）HBV-LP S/A值与HBV-DNA的拷贝数呈正相关性（r=0.959,P＜0.05）.结论 乙型肝炎病毒大蛋白（HBV-LP）能够有效、真实地反映HBV病毒复制情况,可以广泛应用于公共体检领域.

Objective It was to explore the application of the hepatitis B virus large proteins（ HBVLP） in public health physical examination through clinical testing of HBV-LP in Catering practitioners, students and soldiers of New requisition etc. Methods 709 samples of HBV infection people were collected in public health physical examination and all collected samples were detected. ELISA was used to detect HBV serum markers （HBV-M ） and HBV-LP. Real-time PCR was applied to detect quantity of HBV DNA. Results （ 1 ） The detectable results of HBV-LP and HBV DNA were no statistical significance in 709 samples. （ 2 ） The detectable results of HBV-LP and HBV DNA were also no statistical significance in different models of HBV infection patients. The values of x2 and P were respectively 2. 67,0. 60,0. 00 and 0. 10,0.44,1. 00. S/A of HBV-LP and the copies of HBV-DNA had a positive relationship （r = 0. 959 ,P ＜ 0. 05）. Conclusion HBV-LP could reflect the level of hepatitis B virus replication and be used to judge the level of hepatitis B virus replication in people of public health physical examination. HBV-LP may be worth using widely in physical examination.