东亚飞蝗血细胞形态学研究

Morphological study on hemocytes of Locusta migratoria manilensis

通过Wright's染色和光学显微镜对不同地区东亚飞蝗的血细胞进行了观察,发现东亚飞蝗Locusta migratoria manilensis(Meyen)的血细胞包括原血胞、浆血胞、粒血胞和类绛血胞4种类型。原血胞的细胞核为红色,而其它3种血细胞的细胞核均被Wright's染液染成红色和蓝色2种类型。故认为浆血胞是由原血胞分裂而来;浆血胞进一步分化为蓝核浆血胞和红核浆血胞,蓝核浆血胞进一步演化为蓝核粒血胞和蓝核类绛血胞;红核浆血胞进一步演化为红核粒血胞和红核类绛血胞。粒血胞又分化为大颗粒粒血胞和小颗粒粒血胞,小颗粒粒血胞又进一步分化为密集小颗粒粒血胞和稀少小颗粒粒血胞,由此将东亚飞蝗的血细胞分为4类11种。该研究不仅为蝗虫形态学提供依据,同时也为蝗虫生理、蝗虫免疫,以及防蝗治蝗提供依据。

Light microscopy and the Wright stain method revealed four types of hemocytes;prohaemocytes,plasmatocytes,granulocytes and oenocytoids,in Locusta migratoria manilensis （Meyen）.Prohaemocytes’ had a red nucleus,whereas the nucleus of the other three haemocytes was dyed red and blue by Wright’s dye.Plasmatocytes are considered to be disintegrated prohaemocytes and can be further differentiated into red-nucleus plasmatocytes and blue-nucleus plasmatocytes.Blue-nucleus plasmatocytes evolve into blue-nucleus granulocyte and oenocytoids whereas red-nucleus plasmatocytes evolve into red-nucleus granulocyte and oenocytoids.Granulocytes are differentiated into large-granular granulocytes and small-granular granulocytes,the latter are differentiated into exiguous and dense small granular granulocytes.Hemocytes of L.m.manilensi can therefore be divided into four main types with eleven subtypes.This study not only provides a basis for grasshopper morphology,but also grasshopper physiology,immunity and locust control.