红霉素、阿仑膦酸钠抑制磨损颗粒对小鼠巨噬细胞分泌白细胞介素1,6及肿瘤坏死因子α的影响

Erythromycin and alendronate inhibition of wear particles influences macrophage secretion of interleukin 1,interleukin 2,and tumor necrosis factor alpha

背景:阿仑膦酸钠的抗骨吸收作用是通过其对破骨细胞的抑制作用完成的,近年亦有报道证实红霉素有直接抑制破骨细胞的作用。目的:观察阿仑膦酸钠和红霉素抑制钛颗粒刺激巨噬细胞分泌肿瘤坏死因子α、白细胞介素1,6的作用。方法:分离、培养小鼠腹腔巨噬细胞,14h后分为红霉素组及阿仑膦酸钠组,每组分为6个亚组。红霉素组:A组:仅为巨噬细胞;B组:巨噬细胞+钛颗粒;C组:巨噬细胞+钛颗粒+红霉素1μg/L;D组:巨噬细胞+钛颗粒+红霉素10μg/L;E组:巨噬细胞+钛颗粒+红霉素100μg/L;F组:巨噬细胞+钛颗粒+红霉素1000μg/L。阿仑膦酸钠组分组及剂量同红霉素组。培养24h后,用酶联免疫法检测细胞培养上清液中白细胞介素1,6及肿瘤坏死因子α的质量浓度。结果与结论:B组白细胞介素1,6及肿瘤坏死因子α的质量浓度明显高于其他组(P<0.05),F组白细胞介素1,6及肿瘤坏死因子α的质量浓度明显低于C组(P<0.05)。同剂量阿仑膦酸钠和红霉素组间差异无显著性意义(P>0.05)。提示钛颗粒可以刺激巨噬细胞分泌大量的白细胞介素1,6及肿瘤坏死因子α,红霉素、阿仑膦酸钠能够呈剂量依赖型地有效抑制钛颗粒诱导的巨噬细胞分泌白细胞介素1,6及肿瘤坏死因子α。

BACKGROUND：Alendronate has an anti-bone resorption effect which is realized through its inhibitory effect on osteoclasts.Recently,it is confirmed that erythromycin plays a direct role against osteoclasts.OBJECTIVE：To detect the effects of erythromycin and alendronate inhibited titanium（Ti） particles on interleukin 1（IL-1）,IL-6 and tumor necrosis factor alpha（TNF-a） secreted from macrophages.METHODS：Mouse peritoneal macrophages were separated and cultured,and then divided into erythromycin and alendronate groups.Each group was then divided into six subgroups.Erythromycin group：Group A was treated with macrophages alone,group B with macrophage＋Ti particles,group C with macrophage＋Ti particles＋erythromycin（1μg/L）,group D with macrophage＋Ti particles＋erythromycin（10 μg/L）,group E with macrophage＋Ti particles＋erythromycin（100 μg/L）,group F with macrophage＋Ti particles＋erythromycin（1 000 μg/L）.The subgroups and dose of the alendronate group was the same as the erythromycin group.After 24 hours,the levels of IL-1,IL-6 and TNF-a in culture supernatant was detected by enzyme-linked immunosorbent assay.RESULTS AND CONCLUSION：The levels of IL-1,IL-6 and TNF-a were much higher in groups B than those in other groups（P 0.05）.The levels of IL-1,IL-6 and TNF-a were much lower in groups F than those in groups C（P 0.05）.The same dose of alendronate and erythromycin were not statistically significant（P 0.05）.It was shown that the Ti particles could stimulate macrophages to excrete a great amount of TNF-a,IL-l and IL-6.Eythromycin and alendronate could significantly inhibit the production of IL-1,IL-6 and TNF-a secreted by Ti particles induced macrophages in a dose dependent manner.