日本蟳4个野生群体遗传多样性的微卫星分析

Microsatellite analysis of genetic diversity in 4 wild populations of Charybdis japonica

利用10对微卫星引物对我国大连黑石礁(DL)、莱州湾(LZ)、青岛鳌山湾(QD)、海州湾(HZ)4个日本蟳野生群体的遗传多样性进行了分析。结果表明,不同引物获得的等位基因数从11～17不等,10个位点其平均等位基因数为13.600 0,平均有效等位基因数为8.592 0;各位点的平均观测杂合度(Ho)为0.318 2～0.858 4,平均期望杂合度(He)为0.846 6～0.923 9;平均多态信息含量(PIC)为0.828 0～0.914 0,说明各位点在4个日本蟳野生群体内均表现出很高的遗传多样性水平。各群体间遗传分化较大,基因分化指数(FST)为0.032 74～0.088 03。群体间遗传相似性系数、遗传距离及UPGMA聚类分析表明,鳌山湾与海州湾群体亲缘关系最近,而海州湾和莱州湾群体亲缘关系最远。

Genetic diversity of 4 wild populations（Laizhou,Haizhou,Dalian,Qingdao）of Charybdis japonica was assessed by 10 microsatellite markers.Results showed that the number of averages allele of 10 loci was 13.600 0 and alleles obtained from different primers ranged from 11 to 17,the number of average effective alleles was 8.592 0;Observed heterozygosity value（Ho）was 0.318 2 to 0.858 4 and expected heterozygosity value（He）was 0.846 6 to 0.923 9;Average polymorphism information contents was 0.828 0 to 0.914 0,explained high level of genetic diversity in 4 wild stocks.Coefficient of gene differentiation FST ranged from 0.032 74 to 0.088 03,each group had larger genetic differentiation.Genetic identity（I）,genetic distance（D） and cluster dengrogram between 4 stocks of C.japonica showed that Qingdao Bay was closest relative with Haizhou Bay stock,but Haizhou Bay was farthest relative with Laizhou Bay stock.