摘要
目的:探讨脂肪来源的干细胞(ADSC)移植促进大鼠脑缺血后微血管生成作用的可能机制。方法:108只成年雄性Sprague-Darley大鼠,随机分为假手术组(Sham组)、局灶性脑缺血组(MCAO组)、溶剂对照组(vehicle组)和ADSC治疗组(ADSC组),采用改良的Longa线栓法制作大脑中动脉栓塞(MCAO)模型,ADSC移植前用CFSE标记,ADSC组于造模成功1 d后经侧脑室注射入30μL的ADSC细胞悬液,内含1×106细胞,vehicle组则注射同等剂量的PBS。术后4d、7 d和14 d分批断头取脑,检测缺血区脑组织TGF-β1表达的变化。结果:ADSC组术后4 d、7 d和14 d脑组织中TGF-β1的mRNA和蛋白表达水平均较MCAO组和vehicle组明显增高,同时显示脑缺血后经侧脑室移植的ADSC能够存活并分泌生长因子TGF-β1。结论:ADSC移植促进脑缺血大鼠缺血区微血管生成的机制可能与促进TGF-β1的表达有关。
AIM： To explore the mechanisms of adipose-derived stem cells（ADSCs） transplanting induced angiogenesis in rat brain after focal cerebral ischemia.METHODS： 108 male adult Sprague-Dawley rats were randomly assigned into 4 groups： sham-operated group,middle cerebral artery occlusion（MCAO） group,vehicle group and MCAO＋ADSCs-treated group.Rat＇s focal cerebral ischemia model was established by right middle cerebral artery occlusion（MCAO） with modified Longa＇s method.ADSCs were pre-labeled by CFSE before the transplantation into the rat brain.At 1 d after MCAO,30 μL cell suspension which contained 1×10^6 ADSCs was injected into the lateral ventricle of MCAO＋ADSCs-treated rats,and the same dose of PBS was given to the rats of vehicle group as control.At 4 d,7 d and 14 d after MCAO,rats were decapitated to detect the TGF-β1 expression in the infarct area.RESULTS： The expression of TGF-β1 in brain tissues in MCAO＋ADSCs-treated group was significantly higher than MCAO group and vehicle group at 4 d,7 d and 14 d after MCAO,respectively.After transplantation into MCAO rats,ADSCs could survive and express TGF-β1 in the ischemic brain.CONCLUSION： These data suggest that ADSCs transplantation can promote revascularization in cerebral ischemic rats,partly by promoting TGF-β1 expression in the brain.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第8期872-875,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
福建省自然科学基金项目(2008J02822008J0283)
