急性冠脉综合征患者外周血中Foxp3^+调节性T细胞的变化

Changes of Foxp3^+ regulatory T cells in patients with acute coronary syndrome

目的:探讨急性冠脉综合征(ACS)患者外周血Foxp3+(包括CD4+Foxp3+和CD4+CD25+Foxp3+)调节性T细胞(Treg)的变化与意义。方法:分别采用流式细胞术(FCM)、实时定量PCR和酶联免疫吸附法(ELISA)检测44例ACS患者,20例稳定性心绞痛(SA)患者和24例对照组患者外周血Treg细胞百分率,转录因子Foxp3的mRNA表达和血浆TGF-β1的浓度。结果:与对照组患者和SA组患者相比,ACS组患者外周血中CD4+Foxp3+、CD4+CD25+Foxp3+Treg细胞的百分率,Foxp3 mRNA的表达和血浆TGF-β1浓度明显降低(P<0.05),而CD4+CD25+Treg细胞的百分率在三组之间并无显著性差异。结论:ACS患者外周血Foxp3+Treg数量和/或功能的下调,Foxp3+Treg的变化可能与斑块的不稳定密切相关。

AIM： To investigate the changes of Foxp3^＋（including CD4^＋Foxp3^＋ and CD4^＋CD25^＋Foxp3^＋） regulatory T cells（Tregs） in patients with acute coronary syndrome（ACS）.METHODS： Forty-four ACS patients,twenty patients with stable angina（SA） and twenty-four control patients were enrolled.The percentages of the peripheral Tregs,Foxp3 mRNA and plasma level of TGF-β1 were measured by flow cytometry,real-time quantitative PCR and ELISA.RESULTS： The percentages of CD4^＋CD25^＋Foxp3^＋ Tregs and CD4^＋Foxp3^＋ Tregs,Foxp3 mRNA and plasma level of TGF-β1 significantly decreased in the ACS group as compared with those in the SA group and the controls（P〈0.05）,while there was no significant difference in the percentage of CD4^＋CD25^＋ Tregs among all three groups.CONCLUSION： There are less Foxp3^＋Tregs,perhaps with impaired function,in ACS patients,which might contribute to plaque destabilization.