TGF-β在氩激光诱导大鼠脉络膜新生血管中的表达

Expression of TGF-β in choroidal neovascularization induced by argon laser in RWH rats

目的:评价血管内皮转化生长因子-β(transforming growth factor-beta,TGF-β)在氩激光诱导RWH大鼠脉络膜新生血管(CNV)形成过程中的表达及其意义。方法:RWH大鼠21只,随机分成3组,每组7只。单眼视网膜氩激光光凝诱导CNV形成后,分别在光凝后1,2,3wk摘除双眼球,应用免疫组织化学技术对光凝区进行因子Ⅷ相关抗原(FⅧR∶Ag)的检测以观察CNV形成,并检测CNV形成过程中TGF-β的表达,分析其变化趋势。结果:FⅧR∶Ag免疫组织化学检查结果显示,光凝后1wk开始形成CNV,3wk达到高峰。正常RWH大鼠视网膜中,TGF-β在视网膜神经节细胞层、内核层、色素上皮层、视网膜和脉络膜血管内皮细胞表达。视网膜光凝后,除上述部位外,TGF-β在外核层和脉络膜的光凝损伤区阳性表达。光凝后1～3wk,光斑区内FⅧR∶Ag和TGF-β阳性染色密度逐渐增加(P<0.05),FⅧR∶Ag与TGF-β阳性染色密度呈正相关(r=0.83,P<0.05)。结论:CNV形成与TGF-β表达正相关,TGF-β表达增多可能是CNV形成和增殖的主要原因之一。

AIM： To study the expression of vessel endothelial transforming growth factor-β （ TGF-β ） in choroidal neovascularization （CNV） induced by argon laser in RWH rats. METHODS： Three groups of 21 RWH rats were photocoagulated by argon laser in single eye. 1 week, 2 weeks, 3 weeks after photocoagulation, the expression of TGF-β, and factor U related antigen （F Ⅷ R ： Ag） was observed by immunohistochemical method. RESULTS： The result of FUR： Ag immunohistochemistry showed that CNV initially formed 1 week after photocoagulation and reached the peak till 3rd week. TGF-β expression was observed in the ganglion cells, inner nuclear layer, retinal pigment epithelial cells, the vascular endothelial cells of retina and choroid in normal rats. Besides the above part, TGF-β expressed positively in the outer nuclear layer and choroid after retinal photocoagulation. 1 week to 3 weeks after photocoagulation, the positive stained density of F Ⅷ R： Ag and TGF-β in faculous region gradually increased （ P 〈 0.05）. The expression of FUR： Ag was positively correlated with that of TGF-β（r=0.83,P〈0.05）. CONCLUSION： CNV is positive correlated with the expression of TGF-β. The increased expression TGF-β may contribute to the main reason for the formation and development of CNV.