正畸力作用下炎性牙周组织中白介素-8的表达

Effect of orthodontic force on interleukin-8 expression in the inflammatory periodontal tissues

目的:探讨白介素-8(IL-8)在炎性牙周组织条件下参与牙移动表达的变化规律及牙周组织改建的机制。方法:选用35只雄性SD大鼠,空白对照组5只,其余30只大鼠建立实验性牙周炎模型4周后,去除牙周刺激物后再随机分为6组,分别为炎症对照组,炎症加力1、3、5、7、14天组,每组5只。炎症加力组大鼠上颌一侧第一磨牙与上颌切牙之间安置正畸装置,并施加0.49N近中向正畸力移动第一磨牙。在加力后0、1、3、5、7、14天取材,进行组织形态分析法、免疫组化染色检测I L-8在牙周组织中表达的阳性细胞的面密度值(area density,AD)。结果:空白对照组牙周组织内IL-8表达呈弱阳性,炎症对照组牙周组织内IL-8的表达增强。在牙周受压后,炎症加力1、3、5、7天时,第一磨牙近中压力侧牙周膜组织内IL-8的表达与牙周炎大鼠对照组比较,表达强度高,差异有统计学意义,并且IL-8表达的面密度值在5天时达到峰值,14天时IL-8在牙周组织内的表达继续下降,但强度高于正常对照组。结论I:L-8参与了牙周组织的改建,其表达随时间呈一定的规律;在炎症及正畸力联合效应下,牙周组织的炎症反应程度加重,但不会导致牙周组织的严重破坏。

Objective To investigate interleukin-8（IL-8） under the condition of participation in the inflammatory periodontal condition expression changes of tooth movement and periodontal tissue remodeling mechanism.Methods Selected 35 male SD rats,control group 5,and remaining 30 rats with experimental periodontitis model established after 4 weeks after removal of periodontal stimuli were randomly divided into 6 groups,respectively the control group were inflammation,inflammation afterburner 1,3,5,7,14d group,n=5.Afterburner rats maxillary first molar and the maxillary incisors on one side between the placement of orthodontic divices,and applied to the orthodontic force 0.49N past.1,3,5,7,14d after the afterburner derived,organized morphological analysis,immunohistochemistry detected IL-8 expression in periodontal tissues in the face of positive cell density（area density,AD）.Results The periodontal tissue expression of IL-8 in control group was weakly positive,and the periodontal tissue inflammation control expression of IL-8.Compression of the periodontal inflammation afterburner 1,3,5,7d,the first molar in the pressure side of periodontal tissue expression of IL-8 in the control group with periodontitis in rats,the expression of strength,the difference statistically significant,and the expression of IL-8 in the 5d density reached the peak value.When the IL-8 expression in periodontal tissues continued to decline at 14d,but the strength is higher than the normal control group.Conclusion IL-8 participate in the periodontal tissue remodeling,and the expression was a certain time rule.The degree of periodontal tissue inflammation increased by inflammation irritation and orthodontic force,but it does not cause serious damage to the periodontal tissue.