基质金属蛋白酶-9启基因启动子区单核苷酸多态性与卵巢癌易感性的关系

Association of the SNP in the Matrix Metalloproteinase-9 Promoter Polymorphism with Susceptibility of Ovarian Cancer

目的:研究基质金属蛋白酶-9(MMP-9)与中国北方妇女卵巢癌遗传易感性的关系。方法:采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法,检测130例上皮性卵巢癌患者和159例对照组妇女的MMP-9基因启动子区-1562C/T单核苷酸多态性位点的基因型。结果:卵巢癌患者组和对照组的MMP-9基因型分布均符合Hardy-Weinberg平衡(P>0.05)。卵巢癌患者MMP-9中C和T等位基因频率在病例组和对照组分别为89.23%、10.77%及91.82%、8.18%,两组差异无统计学意义(x^2=1.14,P=0.29):病例组C/C、C/T和T/T基因型频率分别为80.00%、18.46%和L 54%,对照组分别为83.65%、16.15%和0%,两者差异亦无统计学意义(x^2=0.65,P=0.42)。与C/C基因型相比,携带T等位基因未能明显增加卵巢癌的发病风险,经年龄校正的OR值为1.28(95%CI=0.7～2.10)。结论:MMP-9启动子区-1562C/T多态性可能与卵巢癌的发病风险无关。

Objective： The aim of this study was to investigate the association of the MMP-9 SNP with susceptibility of ovarian cancer in North Chinese. Methods：Genotypes of the MMP-9 promoter SNP were performed in 130 ovarian cancer patients and 159 healthy controls by polymerase chain reaction-restriction fragment length polymorphism.Results：The MMP-9 genotype distribution in both ovarian cancer patients and healthy controls did not significantly deviated from that expected by Hardy-Weinberg equilibrium （P〉0.05）. The frequency of the C and T allele among ovarian cancer patients and healthy controls were 89.23%, 10.77%,91.82 %,8.18%, respectively. No significant difference in MMP-9 allele distribution was shown between cases and controls（X^2= 1.136,P=0.287）. The genotype frequencies of C/C,C/T,T/T in the case were 80%,18.46% and 1.54%. While the genotype frequencies of C/C,C/T,T/T in the control were 83.65%,16.15% and 0%. No significant difference of genotype distribution was observed between ovarian cancer patients and healthy women（x^2=0.645,P=0.422）. Compare with the C homozygotes, carriers oftbe T allele was not significantly modified the risk of developing ovarian cancer, the adjusted odds ratio was 1.279（95%CI= 0.78-2.096）. Conclusions：MMP-9 promoter SNP was not association with risk of ovarian cancer.