桂皮醛影响K562细胞生长的机理研究

Mechanism of Cinnamic Aldehyde Inhibiting Proliferation of K562 Cells

目的:探讨桂皮醛对慢性髓细胞白血病细胞株K562生长的影响及其机制。方法:以不同浓度的桂皮醛作用于体外培养的K562细胞,CCK-8法检测细胞增殖活性,流式细胞术检测细胞周期、凋亡率Caspase 3表达。结果:桂皮醛呈时间和剂量依赖性影响K562细胞增殖,使细胞周期受阻于G2/M期,并诱导K562细胞凋亡。K562细胞凋亡过程中,活化的Caspase 3表达明显增加。结论:桂皮醛使K562细胞周期受阻,并使Caspase-3活化诱导细胞凋亡,从而实现对K562细胞的生长抑制。

Objective： This study was purposed to investigate effects ofcinnamic aldehyde on the proliferation of chronic myeloid leukemic cell line K562 and its mechanism.Methods：K562 cells were treated by various concentrations of cinnamic aldehyde, The proliferation of K562 cells was detected by CCK-8 assay. Flow cytometry was employed to measure the cell cycle distribution, apoptosis and the expression of active Caspase 3 of K562 cells.Results：Cinnamic aldehyde affected the proliferation and induced the apoptosis of K562 cells in a time- and dose- dependent fashion. Cinnamic aldehyde arrested K562 cells at G2/M. The levels of active Caspase 3 arose in cinnamic aldehyde treated K562 cells. Conclusions：Cinnamie aldehyde inhibited proliferation of K562 cells in vitro via arresting cell cycle and inducing apoptosis. Actived Caspase 3 played an important role in apoptosis ofK562 cells.