PTEN和PI3K/Akt在卵巢浆液性肿瘤中的表达及意义

Expression and significance of PTEN and the PI3K/Akt in ovarian serous tumors

目的:通过检测卵巢浆液性肿瘤(OST)PTEN、PI3K以及Akt磷酸化,探讨OST的发生机制。方法:应用免疫组织化学法,检测5例卵巢正常上皮(NOT)、10例浆液性囊腺瘤(OSA)、18例浆液性交界性肿瘤(OSBT)、38例浆液性癌(OSC)石蜡包埋组织中PTEN、PI3Kp110α和p-AktSer473蛋白的表达,并结合OSC周围组织浸润、淋巴结转移情况进行统计分析。结果:(1)PTEN蛋白在OSC中的阴性率高于OSBT、OSA和NOT,差异有统计学意义(P<0.05);(2)PTEN蛋白与OSC浸润及淋巴结转移的关系均无统计学意义(P>0.05);(3)PI3Kp110α蛋白表达的阳性率在OSC和OSBT中高于OSA和NOT,差异有统计学意义(P<0.01),p-AktSer473蛋白表达的阳性率在OSC和OSBT中高于OSA和NOT,差异具有统计学意义(P<0.05);(4)PI3Kp110α和p-AktSer473蛋白与OSC浸润及淋巴结转移的关系均无统计学意义(P>0.05);(5)在OSC中,PTEN和PI3Kp110α、PTEN和p-AktSer473蛋白表达间均为负相关(P<0.05),而PI3Kp110α与p-AktSer473蛋白表达间为正相关(P<0.05)。结论:PTEN蛋白表达的缺失伴随PI3K/Akt信号通路过度激活,可能共同参与OST的发生、发展。

Objective：Serous ovarian tumors（OST） by detecting PTEN,PI3K and Akt phosphorylation to approach the mechanism of OST. Methods：Immunohistoche-mical method to detect 5 cases of normal ovarian epithelial（NOT）,10 serous cystadenoma（OSA）,18 serous borderline tumors（OSBT）,38 serous carcinoma（OSC） paraffin-embedded tissue in,PTEN,PI3Kp110α and p-AktSer473 protein expression,and infiltration of surrounding tissue with OSC,lymph node metastasis were analyzed.Results：（1） PTEN protein in OSC higher than in the negative OSBT,OSA,and NOT,the difference was significant（P0.05）;（2） PTEN protein and OSC invasion and lymph node metastasis were not statistically significant（P0.05）;（3） PI3Kp110α positive rate of protein expression and OSBT in OSC than in the OSA and NOT,the difference was significant（P0.01）;p-AktSer473 positive rate of protein expression and OSBT in OSC than in the OSA and NOT,differences statistically significant（P0.05）;（4） PI3Kp110α and p-AktSer473 protein OSC invasion and lymph node metastasis were not statistically significant（P0.05）;（5）PTEN and PI3Kp110α,PTEN and p-AktSer473 protein expression is negative correlation in the OSC（P0.05）,but PI3Kp110α and p-AktSer473 protein expression was positively correlated（P0.05）. Conclusion：The absence of PTEN protein expression with over-activation of PI3K/Akt signaling pathway may participate in OST of the occurrence and development.