M胆碱能药物对家兔虹膜M受体亚型的作用

Effect of M cholinergic drugs on rabbit iris M receptor subtype

目的比较不同的Ｍ受体激动剂或Ｍ受体亚型拮抗剂对［３Ｈ］ＱＮＢ与家兔虹膜中Ｍ受体竞争结合的影响。方法放射性配基受体结合试验。结果Ｍ受体激动剂和Ｍ受体亚型拮抗剂均可抑制［３Ｈ］ＱＮＢ与家兔虹膜中Ｍ受体的结合，呈剂量依赖性。激动剂对其抑制的强度依次为：丁公藤碱＞乙酰奎宁＞毛果芸香碱，亚型拮抗剂对其抑制的强度依次为：４ＤＡＭＰ＞ＰＺ＞ｇａｌａｍｉｎｅ。结论ｍ３受体亚型在兔缩瞳的药理作用中起重要作用。

AIM To compare effects of the M receptor subtype agonists and antagonists on the specific QNB binding to the M receptor subtype of rabbit iris. METHODS Radioligand receptor binding assay. RESULTS All agents inhibited the specific binding between QNB and M receptor of rabbit iris. The more the dose increased, the more the effect increased. The order of inhibitory potency of M receptor agonists is erycibele alkaloid>aceclidine>pilocarpine, the order of antagonists is 4 DAMP>pirenzepine>gallamine. CONCLUSION M 3 receptor subtype is predominant in miosis effect of rabbit.