葛缕酮的气道扩张作用和呼吸道抗过敏作用

Effect of bronchodilation and antianaphylaxis of carvone

目的观察留兰香油中葛缕酮的气道扩张作用和对呼吸道介质的影响。方法用豚鼠药物引喘法、豚鼠离体气管片法、致敏豚鼠肺组织ＳＲＳＡ释放和拮抗ＳＲＳＡ、致敏豚鼠离体气管ＳｃｈｕｌｔｚＤａｌｅ反应法检测。结果葛缕酮对豚鼠药物性哮喘具有保护作用，灌胃给药延长５０％剂量为７６ｍｇ·ｋｇ－１，气雾给药为６３ｇ·Ｌ－１；对豚鼠离体气管有直接松弛作用，ｐＤ２值为４２７±００８，并有抗氨甲酰胆碱作用；能抑制致敏豚鼠肺组织ＳＲＳＡ的释放，ＩＣ５０为１８ｍｇ·Ｌ－１，拮抗ＳＲＳＡ收缩回肠的ＩＣ５０为２７ｍｇ·Ｌ－１，并能抑制致敏豚鼠离体气管的ＳｃｈｕｌｔｚＤａｌｅ反应。结论葛缕酮具有气道扩张作用和呼吸道抗过敏作用。

AIM To observe the action of bronchodilation and antianaphylaxis of carvone extracted from M viridis L. METHODS To detect in methods of histamine and acetylcholine induced asthma attacks in guinea pig, isolated guinea pig tracheal smooth muscle experiment, release of SRS A in the lung fragments of sensitized guinea pig and inhibit the SRS A induced ileum contraction, tracheal Schultz Dale release experiment. RESULTS Carvone could protect histamine and acetylcholine induced asthma in guinea pig, the does of 50% prolongation was 7 6 mg·kg -1 when given ig and 6 3 g·L -1 when aerosol. It had relaxant effect on isolated guinea pig tracheal smooth muscle preparations, and the pD 2 values was 4 27±0 08. The drug not only significantly inhibited antigen induced release of SRS A in the lung fragments of sensitized guinea pig (IC 50 18 mg·L -1 ), but also inhibited SRS A induced contraction of the guinea pig ileum (IC 50 27 mg·L -1 ). It could inhibit tracheal Schultz Dale release in the sensitized guinea pigs. CONCLUSION These results show that carvone has effects of bronchodilation and antianaphylaxis.