ELISA法对神经生长因子在大鼠体内药代动力学的研究

Pharmacokinetics of nerve growth factor(NGF)in Rats determined with enzyme immunosorbent assay method

目的研究神经生长因子（ＮＧＦ）在大鼠体内的药代动力学。方法制备兔抗ＮＧＦ抗体，用双抗体夹心酶联免疫吸附实验（ＥＬＩＳＡ）法测定大鼠血浆ＮＧＦ浓度。结果大鼠ｉｖ３２μｇ·ｋｇ－１后血药浓度时间曲线符合二室开放模型，Ｔ１／２α为０３３０ｈ，Ｔ１／２β为１５ｈ。大鼠ＮＧＦｉｍ３２μｇ·ｋｇ－１后血药浓度时间曲线符合一室一级吸收，血浆ＮＧＦ浓度达峰时间为３７１ｈ，Ｔ１／２（Ｋｅ）为４３０ｈ，２４ｈ吸收百分率为６９１％。结论ＮＧＦ肌注吸收良好，血浆ＮＧＦ浓度可在较长时间内维持在有效浓度以上。

AIM To investigate the pharmacokinetics of nerve growth factor (NGF) in rats. METHODS Double antibody sandwich enzyme immunosorbent assay was used to determine the NGF concentration in blood plasma. RESULTS It was found that the characteristics of the plasma drug concentration time curve fit the two compartment model after iv NGF in rats. The half life time was 0 330 hours for distribution phase and 1 54 hours for elemination phase. After im NGF in rats, the characteristics of the plasma drug concentration time curve fit to a one compartment open model with a first order absorption. The peak time was 3 71 hour, the T 1/2( K e) was 4 30 hours. The absorb percentage of im NGF within 24 hours was 69 1%. CONCLUSION NGF was absorbed mostly and could keep effective concentration for a long time after im.