摘要
用６ｍｏｌ／Ｌ盐酸于１１０℃条件下水解饲料添加剂——叶酸，使之游离出谷氨酸，用氢氧化钠中和调节ｐＨ到２，氨基酸分析仪测定谷氨酸含量，经与标准叶酸水解样品比较，计算出叶酸的纯度。该方法重现性好，变异系数ＣＶ＝０．０８％，平均回收率为９８．３４％，浓度与峰面积呈线性相关，相关系数ｒ＝０．９９８７，可随氨基酸分析同时进行，不需改变任何分析条件。
Glutamic acid was dissociated from folic acid by hydrolysis at 110℃ and 6mol/L HCL.PH was adjusted to 2 with NaOH.Glutamic acid was analysed with amino acid analyzer. Folic acid content was calculated by comparing with the standard hydrolysate of folic acid. The method had good reproducibility with variety coefficiant CV=0.08%,average yield of recovery 98.34%,conccntration and area relative cocfficient r=0.9987. Folic acid could analysed with amino acid analyzer without changing any analysis conditions used for determing other amino acid.
出处
《氨基酸和生物资源》
CAS
1999年第2期27-29,共3页
Amino Acids & Biotic Resources
关键词
氨基酸分析
叶酸
饲料添加剂
amino acid analysis
folic acid
glutamic acid
