创伤性骨折IL-6的表达与T细胞亚群的临床关系

The Clinical Relationship Between IL-6 Expression and T Cell Subgroup in Traumatic Fracture

目的研究细胞免疫功能和细胞因子白细胞介素６（ＩＬ－６）在创伤及修复过程中的作用与临床的关系。方法观察１４例重度和２８例轻度创伤患者Ｔ细胞体外培养增殖反应、Ｔ细胞亚群的变化与ＩＬ－６的表达。结果５７．１４％的重度创伤患者Ｔ细胞增殖反应减弱，ＩＬ－６的表达受到抑制，Ｔ细胞体外培养液中ＩＬ－６含量降低为８２．３６ｐｇ／ｍｌ，而轻度创伤和健康对照组分别为１８３．０７ｐｇ／ｍｌ和４７２．９０ｐｇ／ｍｌ，其差异有非常显著性意义。重度创伤患者ＣＤ８＋细胞数量相对增加，百分率增高为３３．４３％，使ＣＤ４＋／ＣＤ８＋比值下降（１５８），明显低于轻度创伤（１．７３）和健康对照组（１．７６），差异有显著性意义。细胞培养液中ＩＬ－６的含量与ＣＤ８＋细胞数量呈负相关（ｒ＝－０．４３８）。结论在重度创伤患者ＣＤ８＋细胞数相对增多时，可抑制Ｔ细胞的增殖能力，并伴有Ｔ细胞体外培养ＩＬ－６表达作用的减弱。

Objective TO study the cellullar immunity function and the role of interleukin-6(IL - 6) during trauma and its recovery stage. Methods 14 cases of serious and 28 mild traumatic fractureswere selected to observe the change of T cell proliferation reaction and IL - 6 presentation of concentration inthe liqUid of T cell culture in vitro. Results 57.14% of serious cases presented the depression of cellproliferation, and IL - 6 concentration reduced to 82. 36 pg/ml, which was lower than that in mild traumagroup (183. 07 pg/ml) and control group(472. 90 pg/ml) respectively. There is statistical significance between the two groups. The rate of CD 8 + T cell was increased relatively in the serious group (33. 43% ), andthe ratio of CD 4 + /CD 8 + (1.58 ) was lower than the group of mild trauma (1. 73 ) and control group (1. 76).There is negative relationship between the concentration of IL - 6 in cell proliferation liquid and CD 8 + T cellratio (r = - 0. 438) in those cases. ConcluSion in serious cases, the T cell proliferation was depressed,while the number of CD 8+ cell increased relatively, and IL - 6 expression was decreased.