摘要
目的:建立尿中硫胺素的荧光检测方法。方法:探讨了各种前处理条件包括直接测定、酸解、酶解对尿液中硫胺素测定的影响。利用本文建立的方法通过尿负荷实验对几位志愿者进行硫胺素营养水平分析。结果:尿液经过酸解,酶解后,在碱性铁氰化钾溶液中氧化,荧光法测定。回收率在73.5%～96.2%之间。结论:此方法可同时测定游离态和结合态的硫胺素,结果可靠。适合于尿液中硫胺素的测定。
Objective：To establish a fluorescent method for the determination of thiamine in urine.Methods： The influencing factors of pretreatment for the determination of thiamine in urine such as direct measurement,acidolysis,enzymolysis were discussed.The investigation of thiamine nutritional level of several voluteers was proceeded by urine load experiment.Results： After acidolysis and enzymolysis,urine was oxidized by alkaline potassium ferricyanide and determined by fluorescent method.The recovery range was 73.5%～96.2%.Conclusion： The method can simultaneously determine the free and bound thiamine.It is accurate.It is reliable and suitable for the determination of thiamine in urine.
出处
《中国卫生检验杂志》
CAS
2011年第7期1631-1632,共2页
Chinese Journal of Health Laboratory Technology
关键词
硫胺素
尿液
荧光法
Thiamine
Urine
Fluorescent method
