摘要
目的:建立了沙浴恒温消化—国标法配套试剂盒测定尿碘的方法。方法:参考尿碘国标法,用沙浴恒温消化尿液,国标法配套试剂盒检测。结果:该法最小检出限为4.5μg/L,标准曲线线性相关系数r=-0.9994;分别对碘含量为73.0μg/L、175μg/L、206μg/L的尿碘标准物质进行6次平行测定,与标准值的偏差分别为2.1%、1.1%、2.9%,相对标准偏差分别为3.1%、1.9%、2.0%;加标回收率96.0%~100%;测定100份尿样与国标法对比测定,测定结果无显著性差异。结论:该法简单、准确、可靠,适合基层实验室使用。
Objective:To establish a kit method for determination of urinary iodine with thermostatic sand bath digestion.Methods: To determine urinary iodine using the national standard kit after the thermostatic sand bath digestion.Results: The minimal detection limit of the method was 4.5 μg/L,while the linear correlation coefficient was-0.9994.The iodine contents of 6 parallel determinations of urinary iodine standard material were 73.0 μg/L,175 μg/L and 206 μg/L,and standard deviation values were 2.1%,1.1%,2.9% respectively and relative standard deviations were 3.1%,1.9% and 2.0% respectively;Recoveries were from 96.0% to 100%.There was no significant difference between determination of 100 urine samples and the national standard measurement method.Conclusion: The method is simple,accurate,reliable and suitable for common laboratories.
出处
《中国卫生检验杂志》
CAS
2011年第7期1640-1641,共2页
Chinese Journal of Health Laboratory Technology
关键词
沙浴恒温消化
试剂盒
尿碘
Thermostatic sand bath digestion
Kit
Urinary iodine