高效液相色谱法测定保健食品中的叶黄素

The determination of lutein in health food by HPLC

目的:建立测定保健食品中叶黄素含量的方法。方法:采用无水乙醇对试样进行提取,纯甲醇体系作为流动相,ODS C18柱,高效液相色谱(HPLC)进行分离检测。结果:方法的检出限为0.1μg/g,线性范围在0.3μg/ml～6.2μg/ml之间,RSD为5.9%,平均回收率为92%～105%。结论:该方法具有简便、快速、可靠、容易推广等优点,适用于液体、片剂、粉剂、胶囊、软胶囊样品的测定。

Objective： To establish a method for the determination of lutein in health foods.Methods： The samples were extracted by alcohol,eluted with methanol on an ODS C18 column by HPLC.Results： The detection limit of the method is 0.1 μg/g,the linear range is 0.3 μg/ml～6.2 μg/ml,and the RSD is 5.9%.The average recovery is 92%～105%.Conclusion： The method is simple,fast and reliable.It will provide a reliable analysis method for detecting the concentration of lutein in health foods,including liquid,tablet,powder,capsule and soft capsule samples.