摘要
目的:建立测定保健食品中叶黄素含量的方法。方法:采用无水乙醇对试样进行提取,纯甲醇体系作为流动相,ODS C18柱,高效液相色谱(HPLC)进行分离检测。结果:方法的检出限为0.1μg/g,线性范围在0.3μg/ml~6.2μg/ml之间,RSD为5.9%,平均回收率为92%~105%。结论:该方法具有简便、快速、可靠、容易推广等优点,适用于液体、片剂、粉剂、胶囊、软胶囊样品的测定。
Objective: To establish a method for the determination of lutein in health foods.Methods: The samples were extracted by alcohol,eluted with methanol on an ODS C18 column by HPLC.Results: The detection limit of the method is 0.1 μg/g,the linear range is 0.3 μg/ml~6.2 μg/ml,and the RSD is 5.9%.The average recovery is 92%~105%.Conclusion: The method is simple,fast and reliable.It will provide a reliable analysis method for detecting the concentration of lutein in health foods,including liquid,tablet,powder,capsule and soft capsule samples.
出处
《中国卫生检验杂志》
CAS
2011年第7期1650-1651,共2页
Chinese Journal of Health Laboratory Technology