人类嗜T淋巴细胞病毒Ⅰ型的PCR检测方法研究

Study on the Human T-cell lymphotropic virus type I detected by PCR

目的:建立PCR检测人类嗜T淋巴细胞病毒Ⅰ型的方法。方法:将HTLV-Ⅰ的PX基因转染大肠杆菌DH5a质粒pUC57构建HTLV-Ⅰ检测参考菌株,针对PX基因设计PCR引物,并考察PCR方法的特异性和灵敏度。结果:凝胶电泳检测到PCR扩增PX基因得到的230bp目标片段,且PCR方法具有较高的灵敏度。结论:该PCR可特异、灵敏地检测人类嗜T淋巴细胞病毒Ⅰ型。

Objective：Development of polymerase chain reaction（PCR） for the detection of Human T-cell Lymphotropic virus type I（HTLV-I）.Methods： HTLV-Ⅰreference stain was constructed by transfecting a part of PX gene into the pUC57 plasmid of E.coli（DH5a）.To design PCR primers by aiming at PX gene and examine the specificity and sensitivity of PCR method.Results： The target gene segment with 230 bp,which was generated from PCR amplifying the PX gene,can be detected by the agarose gel electrophoresis.It also proved that PCR has higher sensitivity.Conclusion： PCR is a special and sensitive method to detect the infection of HTLV-I.