摘要
目的:建立PCR检测人类嗜T淋巴细胞病毒Ⅰ型的方法。方法:将HTLV-Ⅰ的PX基因转染大肠杆菌DH5a质粒pUC57构建HTLV-Ⅰ检测参考菌株,针对PX基因设计PCR引物,并考察PCR方法的特异性和灵敏度。结果:凝胶电泳检测到PCR扩增PX基因得到的230bp目标片段,且PCR方法具有较高的灵敏度。结论:该PCR可特异、灵敏地检测人类嗜T淋巴细胞病毒Ⅰ型。
Objective:Development of polymerase chain reaction(PCR) for the detection of Human T-cell Lymphotropic virus type I(HTLV-I).Methods: HTLV-Ⅰreference stain was constructed by transfecting a part of PX gene into the pUC57 plasmid of E.coli(DH5a).To design PCR primers by aiming at PX gene and examine the specificity and sensitivity of PCR method.Results: The target gene segment with 230 bp,which was generated from PCR amplifying the PX gene,can be detected by the agarose gel electrophoresis.It also proved that PCR has higher sensitivity.Conclusion: PCR is a special and sensitive method to detect the infection of HTLV-I.
出处
《中国卫生检验杂志》
CAS
2011年第7期1705-1706,共2页
Chinese Journal of Health Laboratory Technology