巢式逆转录PCR技术检测肝癌组织GPC3 mRNA的表达

Detection of the expression of GPC3 mRNA in the tissue of HCC by nestedRT-PCR

目的:探讨肝细胞癌(hepatocellμLar carcinoma,HCC)中磷脂酰肌醇蛋白聚糖3(Glypican-3,GPC3)mRNA的表达及其对肝癌检测的诊断价值。方法:运用巢式逆转录PCR(nRT-PCR)技术检测76例HCC组织及其癌旁组织,对照组33例,同时应用化学发光法对76例HCC患者和33例对照组的血清甲胎蛋白(AFP)进行检测。结果:在76例HCC组织中GPC3高表达的有68例(89.5%),癌旁组织高表达有的31例(40.8%),对照组只有3例(9.1%)表达增高;在44例AFP阳性的HCC患者中GPC3表达阳性的有41例(93.2%),在32例AFP阴性的HCC患者中GPC3表达阳性的也有27例(84.3%),GPC3与AFP联合检测阳性率为93.4%(71/76);结合病理特征提示GPC3的表达不仅与HCC的病理分级有关,还可能与HCC的进展和转移有关。结论:GPC3可作为一种新的肿瘤标志物用于肝细胞癌的早期临床诊断,其与AFP联合检测可有效提高肝癌的诊断率。

Objective：To investigate the expressions of glypican-3（GPC3） mRNA in hepatocellμLar carcinoma（HCC） tissues and to explore their value in the diagnosis of HCC.Methods： NestedRT-PCR（nRT-PCR） technique was used to detect 76 cases of HCC tissues and its peficancerous tissues as well as the 33 cases in control group.Meanwhile,chemiluminescence method was used to detect the MSAFP in 76 patients with HCC and 33 controls.Results： The relative intensities expression of GPC3 mRNA in HCC,paracancerous,volunteers were 68 cases（89.5%）,31 cases（40.8%） and 3 cases（9.1%） respectively.Expresssion of GPC3 mRNA in 44 HCC with AFP positive findings is 41（93.2%）,meanwhile in 32 HCC with AFP negative findings is 27（84.3%）.The combined detection positive ratio for GPC3 and AFP are 93.4%（71/76）.With the prompt of pathological features,the expression of GPC3 not only relates to pathologic grade of HCC,but also relates to the progress and transfer of HCC.Conclusion： GPC3 mRNA may be used as a marker for the early diognosis of HCC in clinic.Combined detection of GPC3 and AFP can provide valuable information in the diognosis of the HCC.