维甲酸对人喉鳞癌裸鼠移植瘤的治疗观察

The experimental treatment of laryngeal carcinoma with all trans retinoic acid

目的 寻求有效的喉癌辅助治疗方法，研究全反式（ａｌｌｔｒａｎｓ） 维甲酸（ ｒｅｔｉｎｏｉｃ ａｃｉｄ，ＲＡ）在体内的抗肿瘤作用。方法 利用人喉鳞癌瘤系ＰＨＣ３ 制造裸鼠喉癌模型（ 裸鼠１６ 只， 实验治疗组和对照组各８ 只） ，观察ＲＡ治疗荷喉癌裸鼠的疗效，并在光镜及透射电镜下观察ＲＡ 作用后肿瘤组织显微及超微结构，免疫组化检测肿瘤细胞ｃｍｙｃ 蛋白的阳性率。结果 治疗组肿瘤生长明显受到抑制，抑制率达５０％ 以上（ Ｐ＜ ０．０５）。光镜及透射电镜观察发现，ＲＡ 治疗组裸鼠移植人喉癌细胞有一定程度的良性趋向分化。免疫组化检测肿瘤细胞ｃｍｙｃ 蛋白的阳性率，治疗组（３４．０ ％ ±１０．６％ ） 与对照组（６０．５％ ±１５．２ ％） 相比差异有显著性（ Ｐ＜ ０．０１）。结论 维甲酸对裸鼠移植人喉癌有生长抑制及诱导分化作用，且与ｃｍｙｃ 癌基因及蛋白的调控有关。

Objective To investigate whether all trans retinoic acid (RA)has antitumor effect in vivo Methods Sixteen nude mice were transplanted with human laryngeal cell carcinoma PHC3 Eight of them were injected with RA intraperitoneally and others with solvent as the control Response was measured and tumor histopathological features were studied Results The growth and weight of RA treated tumors were significantly reduced in comparison with those of the control The growth inhibition rate reached more than 50% in the RA group Studies also showed that RA treated cells became well differentiated There was a significant decrease of c myc protein level in the RA treated tumors as compared with the control Conclusion It is suggested that RA could induce differentiation of human laryngeal carcinoma cells and suppress tumor growth in vivo Regulation of c myc gene or protein may be related to those happening