200例男性不育患者的Y染色体微缺失检测

Detection of Y chromosome microdeletions in 200 infertile men.

目的探讨男性不育患者与Y染色体微缺失之间的关系。方法利用15个Y染色体特异序列标签位点,以多重PCR法检测男性不育患者的Y染色体微缺失情况。结果 200例男性不育患者中共检出Y染色体微缺失7例,缺失率为3.5%。其中单纯A;;ZFc缺失2例,缺失率为1%(2/200);A;;ZFb缺失率为3例,缺失率为1.5%(3/200);单纯A;;ZFa缺失2例,缺失率为1%(2/200),尚未发现联合缺失或大片段缺失患者。精液正常者(对照组)30例未发现Y染色体微缺失。结论 Y染色体微缺失是造成男性不育的常见病因之一。

Objective： To explore the relationship between infertile men and Y chromosomal microdeletions.Methods： Infertile men were screened by multi-polymerase chain reactions with 15 Y chromosome specific sequence tag site labels（STS）.Results： Microdeletions were detected in 3.5%（7/200） infertile men.There were 2 infertile men with Y chromosome microdeletion of AZFc,and the deletion rate is 1%（2/200）,and 3 infertile men with Y chromosome microdeletion of AZFb,and the deletion rate is 1.5%（3/200）,and 2 infertile men with Y chromosome microdeletion of AZFa,and the deletion rate is 1%（2/200）.There wasn＇t Y chromosome microdeletion in normal 30 controls.Conclusion： Y chromosome microdeletion is one of the causes which result in male infertile.