子痫前期患者胎盘上HO-2及TNF-α表达的研究

Placental expression of Ho-2 and tnf-α and their relationship in women with preeclampsia.

目的探讨妊娠高血压疾病子痫前期患者胎盘中HO-2及TNF-α表达及其与妊高病发病机制之间的关系。方法采用免疫组织化学方法测定24例子痫患者和24例正常妊娠妇女(对照组)胎盘中HO-2、TNF-α表达水平,并分析两者相关性。结果 1.HO-2主要表达于血管内皮细胞、平滑肌细胞胞浆及胞膜上、而滋养细胞及间质细胞中表达较少。且HO-2在轻度及重度子痫前期患者表达均低于正常妊娠妇女,两者分别与正常晚孕组比较,轻度子痫前期与正常对照组相比差别无显著性,而重度子痫前期与正常对照组比较,差别有显著性。2.TNF-α主要表达于滋养细胞、血管内皮细胞胞膜中,而间质细胞内表达最少。且TNF-α在轻度及重度子痫前期患者表达均低于正常妊娠妇女,两者分别与正常晚孕组比较,差别均有显著性。3.正常组、PIH组HO-2及TNF-α存在负相关关系(r=-0.536、-0.574)。结论 HO-2可能是胎盘血管内皮细胞的保护性因素,而TNF-α可能引起胎盘血管内皮细胞损伤,可导致血管调节因子失衡,两者存在负相关关系,在妊高病发病过程中起一定作用。

Objective：To investigate the placental expression of Ho-2 and tnf-α women with preeclampia and study their relationship.Methods：Immunohistochemistry was used to examine the levels of Ho-2 and tnf-α 24 controls and 24 preeclampsias.Preeclampsias were divided into mild and severe groups.Results： 1.Ho-2 is mainly expressed in cytoplasm and epicyte of endothelial cell and smooth muscle cell,but is lowly trophocyte and interstitial cell.Level of Ho-2 in controls,mild and severe is lower by lower.Level of Ho-2 in severe group were lower than in comtrols（P0.05）,and no difference between controls and mild group（P0.05）.2.Tnf-α is mainly expressed in cytoplasm and trophocyte and epicyte of endothelial cell,but is lowly interstitial cell and smooth muscle cell.The level of tnf-α in mild and severe group were higher than in controls.（P0.05） 3.There were significantly negative correlations between Ho-2 and tnf-α in mild and severe preeclampsia groups（r=-0.864,P0.05 r=-0.688,P0.05）,but there were no significantly negative correlations between Ho-2 and tnf-α in controls（r=-0.184,P=0.388）.Conclusions： Ho-2 may protect the endothelial of placenta,but tnf-α may damage endothelial of placenta.Ho-2 and tnf-α may contribute to the pathology of preeclampia.